酵母PHO81基因上游调控序列的初步研究  

Studies on the Upstream Requlatory Sequence of Yeast Acid Phosphatase Gene PHO 81

作　　者：李江琪[1] 吴建生[1] 敖世洲[1] 

机构地区：[1]上海生物化学研究所

出　　处：《南昌大学学报（医学版）》1995年第1期1-3,共3页Journal of Nanchang University：Medical Sciences

摘　　要：ＰＨＯ８１基因的表达受无机磷酸盐浓度的调控，属阻遏型基因。利用本组构建的ＰＨＯ８１－ＬａｃＺ融合基因，对ＰＨＯ８１基因５＇上游核苷酸序列作限制性内切酶缺失分析。以β－半乳糖苷酶的活力表示ＰＨＯ８１的表达水平，测定上游长度不同的变种表达ＰＨＯ８１基因的水平，提示ＰＨＯ８１基因起始密码上游可能存在着两个ＵＡＳ功能区。The yeast acid phosphatase requlatory gene PHO 81 is a repressive gene.The expressionof the gene is in response to the presence or absence of exogenous inorganic phosphate. Tostudy its regulatory mechanism,we have sequencde 5 noncoding region 1244bp of the PHO81 gene. The coding region was fused with LacZ gene and the gene expresional level was as-sayed by measuring beta galactosidase activity. Using restriction endonuclease,the 5'-flank-ing sequences of PHO 81 were deleted. The expressional level of various deletion derivativesshowed there were two upstream activating sequences(USA)locating at -402 to -1012 and-289 to -402 upstream of the initiation codon.

关 键 词：基因 调节 酸性磷酸酶 酵母 上游调控序列 

分 类 号：Q786[生物学—分子生物学]