DNA对电穿孔转染效率的影响研究  

The Effect of DNA on Efficiency of Electroporation

作　　者：卢柏松[1] 黄培堂[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》1995年第1期16-20,共5页Letters in Biotechnology

摘　　要：以外源红细胞生成素eDNA的表达产物为指标,研究了运载DNA和重组表达质粒的构象对电穿孔转染CHO细胞的效率的影响。结果250μg/ml的运载DNA可使外源基因表达水平提高3倍;线性化质粒DNA比超螺旋DNA更适合于用电穿孔方法获得永久表达。这一结果提示,运载DNA的存在和质粒DNA的线性化对提高电穿孔转染CHO细胞的效率是必需的。The effect of DNA co (formation and carrier DNA on the efficiency of electroporation are studied using the expression of erythropoietin DNA in CHO cells as a indicator. It turns out that 250μg/ml carrier DNA increases the expression level by 3 fold, and linalized plasmid DNA is more suitable than supercoiled DNA to obtain stable trans-fection by electroporation. The results suggest that it is essential to linalize plasmid DNA and cotranxfect with appropriate carrier DNA to ( btain stable and high level expression of foreign gene by means of electroporation.

关 键 词：电穿孔转染 运载DNA DNA构象 

分 类 号：Q522[生物学—生物化学]