rhGM-CSF cDNA直接注射小鼠骨骼肌的体内表达研究  

Direct rhGM-CSF cDNA Transfer Into Mouse Muscle and Expression in Vivo

作　　者：张述[1] 苏国富[2] 鲍云华[1] 芮贤良[2] 叶棋浓[2] 隋拥君[2] 王恒梁[2] 徐永强[2] 

机构地区：[1]军事医学科学院附属医院肿瘤科 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》1995年第4期158-161,共4页Letters in Biotechnology

摘　　要：本实验将含有人粒-巨噬细胞集落刺激因子(hGM-CSF)基因cDNA的重组真核表达质粒直接注射小鼠骨骼肌,观察了hGM-CSF基因在小鼠体内的分泌表达情况。ELISA结果显示注射重组质粒DNA后,第15天左右小鼠血液hGM-CSF的表达量最高,约有97 ng/ml,第20天次之,第25天和第10天的表达量相近,约有49 ng/ml。生物学活性检测结果表明,实验组小鼠血液有维持TF-1依赖株细胞的生长作用。表明重组质粒DNA直接注射骨骼肌不仅能表达hGM-CSF,而且表达产物有生物学恬性。The plasmid pCG1 which was constructed by the eucaryotic expression plasmid pCDS, was directly transferred into the BAL B/c mice' s quadriceps. The results of ELISA showed that hGM-CSF was expressed and secreted in mice in vivo. The highest expression level of hGM-CSF was about 97ng/ml on the fifteenth day after DNA was injected. Meanwhile,the results of biological activity detection showed that C group mice's serum had the function of retaining the growth of TF-1 cells. This suggested that the hGM-CSF had certain biological function.

关 键 词：基因治疗 HGM-CSF DNA注射 骨骼肌 

分 类 号：R346[医药卫生—基础医学]