应用免疫组化方法检测癌基因ras产物P21在胃癌及癌前组织的表达  

Immunohistochemical detection of ras oncogene product P21 in gastric cancer and its precancerous lesions

作　　者：房殿春[1] 刘为纹[1] 

机构地区：[1]重庆第三军医大学西南医院消化科,630038

出　　处：《世界华人消化杂志》1994年第4期225-226,199,共3页World Chinese Journal of Digestology

摘　　要：目的:对胃癌及癌前组织ras族基因产物P21的表达进行观察,籍以探讨P21异常表达在胃癌友生中的作用。方法:对110例胃癌、32例肠型异型增生及311例胃粘膜肠化组织中的P21进行免疫组织化学染色,并按组织学和粘液染色结果将胃粘膜肠化分为Ⅰ、Ⅱ和Ⅲ型。结果:110例胃癌P21染色阳性45例,阳性率为40.9％,早期癌染色阳性率与中晚期相比无显著差别(P>0.05)。32例异型增生P21染色阳性率为21.9％。在不同类型肠化中,Ⅲ型肠化染色阳性率(23.3％)与Ⅰ型(82％)和Ⅱ型(6.7％)相比差别非常显著(P<0.01)。结论:P21表达异常在胃癌恶变中可能起重要作用,Ⅲ型肠化与胃癌的发生可能有密切关系。AIM The expression of ras P21 in gastric cancer and its precancerous lesions were observed to determine the role ofenhanced P21 expression in the development of gastric cancer.METHODS Immunohistochemical staining of ras P21 was performed on the specimens of 110 gastric cancer,32 dysplasiaand 311 intestinal metaplasia which was classifid into types Ⅰ,Ⅱ and Ⅲ using both morphological and histochemicalcriteria.RESULTS Ras P21 was detected in 45(40.9%)of 110 gastric cancer and in 7(21.9%)of 32 dysplasia.There was nosignificant difference in ras P21 expression between early and advanced gastric cancer.Among the different types ofintestinal metaplasia,type Ⅲ demonstrated significantly higher positivity rate(23.3%)than type Ⅰ(8.2%)and Ⅱ(6.7%)(P<0.01).CONCLUSION The tansformation of the stomach mucosa from benign to malignant phenotype is associated with an increase in ras P21 expression and the development of gastric cancer may be related to the presence of typeⅢ intestinal metaplasia.

关 键 词：胃癌 癌前期病变 ras P21 

分 类 号：R735.2[医药卫生—肿瘤]