CONSTRUCTION AND EXPRESSION OF BACTERIORHODOPSIN MUTANT IN HALOBACTERIUM HALOBIUM  

作　　者：汪俭 梅祺 卢春林 

出　　处：《Journal of Southeast University(English Edition)》1994年第2期38-42,共5页东南大学学报（英文版）

摘　　要：Site-specific mutagenesis of bacteriorhodopsin(bR)is an importanttechnique for studying the niechanism of pruton-pumping and optic-electronic fea-turee of bR molecules.The bop gene niutant with une nucleutide substitutiun of G→A at position 286 is constructed and expressed in Halubacterium HalubiuM L33(bop-).The result of mutation is identified by DNA sequencing, western blottingand absorption spectra analysis.The altered bup gene can be inherited stably in L33cells.ＣＯＮＳＴＲＵＣＴＩＯＮＡＮＤＥＸＰＲＥＳＳＩＯＮＯＦＢＡＣＴＥＲＩＯＲＨＯＤＯＰＳＩＮＭＵＴＡＮＴＩＮＨＡＬＯＢＡＣＴＥＲＩＵＭＨＡＬＯＢＩＵＭＷａｎｇＪｉａｎ（汪俭）；ＭｅｉＱｉ（梅祺）；ＬｕＣｈｕｎｌｉｎ（卢春林）（ＤｅｐａｒｔｍｅｎｔｏｆＢｉ...

关 键 词：BACTERIORHODOPSIN site-spocific mutasenesis gene EXPRESSION H.H.L33 

分 类 号：Q939.44[生物学—微生物学]