内皮素A型受体（ET＿A－R）基因在大鼠心血管系统细胞中的表达  

EXPRESSION OFENDOTHE LINARECEPTOR (ETA一R )GENEINTHE RAT CELLSOFCARIDIOVASCULAR SYSTEM

作　　者：周洪[1] 张晨晖[1] 李倩虹[1] 牛大地[1] 张新波[1] 周爱儒[1] 温进坤[1] 汤健[1] 

机构地区：[1]北京医科大学心血管基础研究所

出　　处：《北京大学学报（医学版）》1994年第S1期29-32,共4页Journal of Peking University:Health Sciences

摘　　要：应用改良的Ｍａｒｔｉｎ（１９９３）定量反转录－聚合酶链式反应（ｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｉｏｎ－ｐｏｌｙ－ｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＲＴ－ＰＣＲ）方法，测定了大鼠ＥＴＡ型受体（（Ｅｎｄｏｔｈｅｌｉｎ－Ａｒｅｃｅｐｔｏｒ，ＥＴＡ－Ｒ）ｍＲＮＡ在心血管系统细胞中的分布。结果证明，ＥＴＡ－ＲｍＲＮＡ广泛地分布在心血管系统细胞中．其中以平滑肌细胞中含量最高。内皮素及其受体的配基可以促进血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈＭｕｓｃｌｅｃｅｌｌ，ＶＳＭＣ）中ＥＴＡ－ＲｍＲＮＡ的表达。此外，还发现在自发性高血压动物的血管平滑肌细胞中ＥＴＡ－Ｒ基因的高表达可能是高血压血管高反应性的一个重要机制。With Reverse Transcription- Polymerase Chain Reaction (RT-PCR ),expression of endothelin- Areceptor(ETA - R) mRNA in the cells of rat cardiovascular system was studied.It was found that(ETA- R) mRNA is widely distributed in the cells of rat cardiovascular system.Among them,(ETA- R)mRNA level is the highest in the smooth muscle cells(SMCs) .Endothlin can significantly increase the(ETA - R) mRNA level in SMC,and in addition,(ETA- R)mRNA levels are significantly increased in spontaneously hypertensive rat VSMC,These data show that the high expression of( ETA - R) gene in SMC may play an important role in the pathogenesis of hypertension.

关 键 词：内皮素A型受体 基因表达 高血压 

分 类 号：R394.3[医药卫生—医学遗传学]