AHTG与柔红霉素的偶联物体外对正常人粒单祖细胞的影响及其抗Molt-4细胞作用  

The Effects of AHTG-Dex-DNR Conjugate on Normal Bone Marrow CFU-GM and Its Anti-Molt-4 Cells Activity in Vitro

作　　者：夏维林[1] 唐锦治[1] 张锦玲[1] 张东华[1] 

机构地区：[1]同济医科大学附属同济医院内科,武汉430030

出　　处：《华中科技大学学报（医学版）》1994年第S2期106-108,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：市科委科技项目课题

摘　　要：本文采用克隆测定法观察抗人胸腺细胞球蛋白与柔红霉素偶联物（AHTG-Dcx-DNR）体外对正常人粒单祖细胞的影响及其抗T淋巴细胞白血病细胞系Molt-4作用。不同浓度偶联物与骨髓细胞共同孵育2h后进行CFU-GM培养，结果表明，含柔红霉素（DNR）0.01mg／L和0．1mg/L的偶联物对正常人骨髓CFU-GM的抑制率较相同浓度的游离DNR小（P＜0.01和P＜0.05），而较高浓度的偶联物（含DNRlmg／L)对CFU-GM则呈一定抑制作用。偶联物处理Molt-4细胞后能有效地抑制白血病集落的形成，呈量效关系，提示该偶联物体外有抗白血病作用。The effects of AHTG-Dex-DNR conjugate on CFU-GM of normal bone morrow and its antitumor activity on T-lymphocytic leukemic cell line Molt-4 in vitro were observed by means of colony assay.The bone morrow cells were incubated with different concentrations of conjugate for 2 h， the CFU-GM culture was performed． The results showed that the inhibition rate on normal bone morrow CFU-GM by the conjugate with 0.01mg／L and 0．1mg／L DNR was significantly less than that of corresponding concentra tions of free DNR(P＜0.01and P＜0.05).However,When the concentration of DNR in the conjugate was increased to l mg／L，the inhibitory effect rose correspondingly.When Molt-4 cells were pre-treated with AHTG-Dex-DNR,the colony forming units of Molt-4 were effectively inhibited.It suggested that the conjugate was cytotoxic against leukemic cells with a dose-dependent effect.

关 键 词：免疫偶联物 粒单造血祖细胞 白血病细胞系 克隆测定 

分 类 号：R733.7[医药卫生—肿瘤]