生长抑素对人胃癌细胞生长的作用及机制初探：与表皮生长因子受体和多胺的关系  

THE ROLE OF SOMATOSTATIN IN THE GROWTH OFHUMAN GASTRIC CANCER CELLS AND ITS MECHANISM: IRELATIONSHIP BETWEEN EPIDERMAL GROWTH FACTOR RECEPTOR AND POLYAMINE SYNTHESIS

作　　者：肖作亮[1] 陈元方[1] 陆国钧 范振符 

机构地区：[1]中国协和医科大学北京协和医院,中国医学科学院肿瘤研究所核医学科

出　　处：《中华消化杂志》1994年第S1期18-21,共4页Chinese Journal of Digestion

摘　　要：本研究对生长抑素（ＳＳ）在人胃癌细胞生长中的作用及机制进行探讨。将培养的人胃癌细胞ＭＧＣ８０及ＳＧＣ７９０１与ＳＳ和／或ｈＥＧＦ（人表皮生长因子）共同孵育后，进行３Ｈ－胸腺嘧啶摄取计数和ＥＧＦ放射受体分析，以观察ＳＳ对人胃癌细胞生长的作用及对ＥＧＦ受体的调节作用，同时探讨ＳＳ对细胞多胺合成的影响。结果发现，ＳＳ能显著抑制两株人胃癌细胞的生长，且能抑制ｈＥＧＦ的促人胃癌细胞生长的作用，并首次发现，ＳＳ对ＥＧＦ受体有下调作用，能使ＥＧＦ受体结合率与对照组相比下降４０％；我们还首次发现，ＳＳ能抑制ＥＧＦ的促细胞多胺合成作用。提示ＳＳ和ｈＥＧＦ在人胃癌细胞生长过程中的相互影响和相互作用，以上结果表明，ＳＳ对人胃癌细胞生长的抑制作用的至少部分是由于它对ＥＧＦ的负性影响，这对阐明肽类激素不同细胞内传导途径间的相互作用，具有重要的理论意义，也为肿瘤的治疗展示了某些新的前景。Recently, the growth factor-like effects or growth-inhibitory effects of some GI hormones have been noticed but their mechanisms and inter-relationship has not yet been elucidated. In this study, the effect of SS on the growth-promoting action of EGF and polyamine synthesis were examined in human gastric cancer cells. The results showed that:(1) SS obviously inhibited the growth of human gastric cancer cells either alone or in the presence of 10-8 M hEGF.(2) SS also down-regulated EGFRs on these cell lines. The specific binding of EGF decreased by 40%.(3) hEGF significantly increased the synthesis of polyamine in human gastric cancer cells(MGC80). SS partialy inhibited the effect of hEGF on polyamine synthesis. Conclusions: SS interacts with hEGF in the regulation of gastric cancer cell growth, either by down-regulating EGF receptors, or by inhibiting EGF-promoted polyamine synthesis. These findings have theoretical implications in elucidating the interactions between different intracellular signal transduction pathways.

关 键 词：生长抑素 表皮生长因子 受体调节 多胺 胃癌细胞 

分 类 号：R735.2[医药卫生—肿瘤]