大鼠更年期实验模型研究  

Experimental Study on Climacteric in Rats

作　　者：程化奇[1] 熊舜华[2] 殷新尤[2] 候安继 杨勇[2] 江仙远 夏振信[2] 张荣[2] 王瑞绵 戎诚兴[2] 

机构地区：[1]湖北医科大学附属第二医院中医科,湖北省武汉市430071 [2]湖北医科大学

出　　处：《实验动物与比较医学》1993年第4期192-196,共5页Laboratory Animal and Comparative Medicine

摘　　要：以11～15月龄雌性SD大鼠为实验组,3～5月龄雌性SD大鼠为对照组,观察血清促性腺激素、性激素水平,阴道脱落细胞涂片,卵巢、子宫、肾上腺皮质光镜变化及心房肌特殊颗粒(ASG)和超微结构的变化。结果发现实验组大鼠阴道细胞涂片为无规则动情周期变化;血清雌二醇(E_2)水平降低、促黄体生成激素(LH)水平升高,促卵泡生长激素(FSH)有升高趋势;卵巢组织切片显示原始卵泡及初、次级卵泡减少,退化卵泡增多,卵巢与子宫内膜变化不相匹配;肾上腺皮质总厚度及束状带变薄,球、网状带增厚,结构出现紊乱并有出血;ASG数目减少,心肌呈衰老趋势。结果提示11～15月龄普通级雌性SD大鼠已进入自然更年期,可用以研究更年期综合症。In experimental group of female SD rats at the age of 11～15 months and con-trol group of those at 3～5 months, the serum levels of gonadotropin and sex hor-mones were detected, smear of vaginal cell, structure of ovary, uterus and adrenalcortex, changes of atrial specific granule (ASG) were observed by microscope andelectron microscope. The results showed no regular estrous cycle in experimentalrats, the levels of serum estradiol (E2) reduced and LH elevated significantly, FSHrose slightly, there is reduction in the number of the primordial, primary andsecondary oogoniums with increase in degenerated oogoniums. The histologicalchanges in the ovary and endometrium were not matched with each other, themyocardium showes the tendency of senile changes. The results suggest that thefemale SD ras at the age of 11～15 months enter their natural climacteric andperhaps it can be adopted as the animal model for human climacterie study.

关 键 词：更年期 大鼠 性激素 性周期 

分 类 号：R[医药卫生] Q95[生物学—动物学]