Investigations of Retention Rule in Reversed-Phase High Performance Liquid Chromatography——Theoretical Consideration of Retention Convergence and Experimental Verification  

作　　者：焦庆才 陈耀祖 师治贤 

机构地区：[1]State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PRC [2]Northwest Plateau Institute of Biology, Academia Sinica, Xining 810000, PRC

出　　处：《Science China Chemistry》1993年第1期1-9,共9页中国科学（化学英文版）

摘　　要：This paper studied the effect of different chromatographic parameters in RP-HPLC (for example,composition of mobile phase, temperature, number of carbon atoms within the solute molecules, solvent molecules and alkyl ligand on bonded phase surface) on the retention convergence based on the displacement adsorption multi-interaction model,and derived a few retention convergent equations by using thermodynamic method,which have been verified by a great deal of experimental data of homologous series.Moreover,we have developed a general method, which can calculate the coordinate values of various retention convergent points by computer directly from experimental data,and the results predicted are in good agreement with that obtained by using plotting method.This paper studied the effect of different chromatographic parameters in RP-HPLC (for example,composition of mobile phase, temperature, number of carbon atoms within the solute molecules, solvent molecules and alkyl ligand on bonded phase surface) on the retention convergence based on the displacement adsorption multi-interaction model,and derived a few retention convergent equations by using thermodynamic method,which have been verified by a great deal of experimental data of homologous series.Moreover,we have developed a general method, which can calculate the coordinate values of various retention convergent points by computer directly from experimental data,and the results predicted are in good agreement with that obtained by using plotting method.

关 键 词：RPLC RETENTION model RETENTION CONVERGENCE HOMOLOGOUS series. 

分 类 号：O6[理学—化学]