Hormonal Regulation of Tissue-type Plasminogen Activator and Plasminogen Activator Inhibitor Type-1 Gene Expression in Cultured Mouse Sertoli Cells  

作　　者：刘以训 杜群 胡召元 Ny Tor 

机构地区：[1]Department of Endocrinology, Institute of Zoology, Academia Sinica, Beijing 100080, PRC [2]State Key Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing 100080, PRC [3]Unit for Applied Cell and Molecular Biology, Umea University, S90187 Umea, Sweden

出　　处：《Science China Chemistry》1993年第3期319-328,共10页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China and Swedish Medical Research Council.

摘　　要：We have demonstrated for the first time that (ⅰ) mouse Sertoli cells predominantly secrete tPA under the action of FSH and cAMP-generating agents, whereas Leydig cells mainly produce uPA; (ⅱ) Sertoli cells are also capable of secreting PAI-1, as well as FSH, growth factors and GnRH increase PAI-1 gene expression; (ⅲ) the increases in tPA and PAI-1 activities by different hormones in the conditioned media of Sertoli cells correspond to the increases in the levels of tPA and PAI-1 mRNA in the cultured cells, suggesting that the synthesis of the activator-inhibitor in mouse Sertoli cells is regulated at a transcriptional level and the tPA secreted by Sertoli cells may be involved in the spermatogenesis.We have demonstrated for the first time that (ⅰ) mouse Sertoli cells predominantly secrete tPA under the action of FSH and cAMP-generating agents, whereas Leydig cells mainly produce uPA; (ⅱ) Sertoli cells are also capable of secreting PAI-1, as well as FSH, growth factors and GnRH increase PAI-1 gene expression; (ⅲ) the increases in tPA and PAI-1 activities by different hormones in the conditioned media of Sertoli cells correspond to the increases in the levels of tPA and PAI-1 mRNA in the cultured cells, suggesting that the synthesis of the activator-inhibitor in mouse Sertoli cells is regulated at a transcriptional level and the tPA secreted by Sertoli cells may be involved in the spermatogenesis.

关 键 词：SERTOLI cells TPA PAL-1 SPERMIATION spermatogenesis. 

分 类 号：O6[理学—化学]