Intermediate Filaments in Higher Plant Cells and Their Reconstitution in vitro  

作　　者：杨澄 邢力 翟中和 

机构地区：[1]Department of Biology, Peking University, Beijing 100871, PRC

出　　处：《Science China Chemistry》1993年第8期940-947,共8页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China.

摘　　要：Delicate networks of 10-nm filaments were found to exist widely in suspension cells of cultured carrot and root tip cells of pea by selective extraction combined with whole mount and diethylene glycol distearate plastic embedment-free section for electron microscopy. These filament components are polypeptides of 52, 58, 62—64 and 50kD using Western blot analysis. A relatively uniform 10-nm filaments, as well as bundle of such filaments could be reconstituted in vitro. The reconstituted filaments were indistinguishable from native plant intermediate filaments in morphology and protein components. Thus the intermediate filaments are actually present in cytoplasm of higher plants. We also found no obvious specificity in tissues and species by comparison of the proteins among various types of plant cells.Delicate networks of 10-nm filaments were found to exist widely in suspension cells of cultured carrot and root tip cells of pea by selective extraction combined with whole mount and diethylene glycol distearate plastic embedment-free section for electron microscopy. These filament components are polypeptides of 52, 58, 62—64 and 50kD using Western blot analysis. A relatively uniform 10-nm filaments, as well as bundle of such filaments could be reconstituted in vitro. The reconstituted filaments were indistinguishable from native plant intermediate filaments in morphology and protein components. Thus the intermediate filaments are actually present in cytoplasm of higher plants. We also found no obvious specificity in tissues and species by comparison of the proteins among various types of plant cells.

关 键 词：INTERMEDIATE FILAMENT selective FRACTIONATION embedment-free section Western BLOT KERATIN FILAMENT assembly. 

分 类 号：O6[理学—化学]