使用悉生大鼠建立肠道膜菌群的分析方法  

ESTABLISHMENT OF EXPERIMENTAL ANALYSIS OF THE INTESTINAL MENBRANEOUS BACTERIAL FLORE BY GNOTOBIOTIC TECHNIC

作　　者：熊德鑫[1] 祝小枫[1] 盛志勇[1] 黎沾良[1] 

机构地区：[1]中国人民解放军三○四医院创伤中心研究室,北京100037

出　　处：《中国微生态学杂志》1993年第2期47-51,共5页Chinese Journal of Microecology

摘　　要：为了深入地研究肠道菌群的生物屏障作用,我们使用悉生动物,建立了肠道膜菌群的研究方法,此法与传统方法相比不仅选择效果好,而且各类菌的活菌计数量高。使用悉生动物的研究结果表明:空肠膜菌群杆菌数为5.09±0.09.肠球菌数为4.49±0.23,乳杆菌数为5.82±0.07,类杆菌数为5.56±0.43,双歧杆菌数为5.19±0.24。回肠膜菌群数依次为6.07±0.29,6.57±0.11,7.40±0.13,6.56±0.09,7.68±0.08。盲肠的膜菌群数依次为6.11±0.45,6.40±0.17,7.04±0.31,7.57±0.22,7.51±0.11。For exploring on the action of biological defference of the host by the intestinal microbiol flora, we establish a new gnotobiotic technic of studying in testinal membraneous flora.This method as comparedwith the conventional one showed better selective action, and a resultof bacterial live count more numerous than that with the ordinary method by using gnotobiotic experimental technics.It was found that the bacterial count inthe jejunum being enterobacter iaceae 5.09±0.09, enterococci 4.49±0.32, lactobacilli 5.82±0.07, bacteroides 5.53±0.43 and bifidobacteria being 5.19±0.24.Whereas the counts in the ileum being respe cfively in above seccession.6.07±0.29, 5.57±0.11, 7.40±0.13, 6.56±0.09, 7.68±0.08; and those in the caecum being in similar seccession.6.11±0.45, 6.40±0.17, 7.04±0.31, 7.57±0.22 7.51±0.11.

关 键 词：肠道膜菌群 肠球菌 大鼠 悉生动物 活菌计数 生物屏障 兼性厌氧菌 需氧培养 需氧菌 稀释法 

分 类 号：Q[生物学] R[医药卫生]