甲硝哒唑对脆弱类杆菌作用机理的研究  

STUDIES ON THE INTERACTIVE MECHANISM OF METRONIDAZOLE AGAINST BACTEROIDES FRAGILIS

作　　者：范昕建[1] 冯萍[1] 汪冰[1] 黄安华[1] 朱淑媛[1] 赵英 王玉芬[2] 王浴生[2] 

机构地区：[1]华西医科大学传染病学教研室,成都610041 [2]华西医科大学基础医学院药理研究室,610041

出　　处：《中国微生态学杂志》1993年第4期1-5,共5页Chinese Journal of Microecology

摘　　要：采用同位素标记前体体外掺入技术和透射电镜观察方法从DNA. RNA及蛋白质水平上进行了甲硝哒唑对脆弱类杆菌作用机理研究。实验表明:甲硝哒唑几乎能完全抑制~3H-TdR掺入脆弱类杆菌,对~3H-亮氨酸的掺入也具明显的抑制作用,未观察到对RNA合成的影响。透射电镜下观察到,随着甲硝哒唑浓度增加,菌体形态发生明显的改变。The interactive mechanism of metronidazole against B. fragills ATCC25285 was studied by incorporation of ~3H-TdR and ~3H-Leu as well as transimission electron microscopy from the level of DNA, RAN and proteins in vitro. From these result, it was inferred that incorporation of ~3H-TdR in B. fragilis was almost inhibited by metronidazole completely, so was ~3H-Leu obviously. Effect of metronidazole on RNA synthesis was not observed. Metronidazole-induced morphological changes in B. fragilis ATCC25285 were also observed apparently by means of a transimission electron microscopy at the higher concentration of metronidazole.

关 键 词：甲硝哒唑 脆弱类杆菌 

分 类 号：Q[生物学] R[医药卫生]