IMMUNOHISTOCHEMICAL DIAGNOSIS IN FINE-NEEDLE ASPIRATION CYTOLOGY  

作　　者：施达仁 王龙富 何公平 冯丽青 范月珍 张仁元 

机构地区：[1]Department of Pathology, Cancer Hospital, Shanghai Medical University, Shanghai 200032 [2]Department of Pathology, Cancer Hospital, Shanghai Medical University, Shanghai 200032his paper reports 25 kinds of polyclonal or monoclonal antibodies by ABC immunohistochemical technique used for 253 cell smears by fine-needle aspiration. The results were,1. Immunohistochemical diagnosis were classified into 136 metastatic cancers ( K12+ EMA+ CEA+ LCA-),92 lymphomas (LCA+ k12- EMA- CEA-), 4 mesenchymal tumors (Vimentin+), 3 melanomas (S-100+NSE+). 15 reactive proliferations (k+λ4+ CD+ CD8+) and 3 unspecified.2. The origin of 70 metastatic cancers were classified into 36 lung (HLC3-AB+), 4 gastrointestinal tract (MG7+), 8 thyroid (TGB+), 1 prostate (PSA+), 3 liver (AFP+) and 14 unknown. 3. Immunologic phenotype of 87 lymphomas wereclassified into 66 cases of B-cell, 4 T-cell, 3 hsitocyte, 7 Hodgkin' s diseases and 7 unclear. The above results suggest that immunohistochemlcal method may be used as a new method of diagnosing and differentiating epithelial and non-epithelial tumors, detecting primary focus of metastatic cncer, differentiating between reactive proliferation adn lymphome and specifying immunologic phenotype of lymphoma in cell smears of fine- needle aspiration.

出　　处：《Chinese Journal of Cancer Research》1993年第2期52-59,共8页中国癌症研究（英文版）

摘　　要：This paper reports 25 kinds of polyclonal or monoclonal antibodies by ABC immunohistochemical technique used for 253 cell smears by fine-needle aspiration. The results were,1. Immunohistochemical diagnosis were classified into 136 metastatic cancers ( K12+ EMA+ CEA+ LCA-),92 lymphomas (LCA+ k12- EMA- CEA-), 4 mesenchymal tumors (Vimentin+), 3 melanomas (S-100+NSE+). 15 reactive proliferations (k+λ4+ CD+ CD8+) and 3 unspecified.2. The origin of 70 metastatic cancers were classified into 36 lung (HLC3-AB+), 4 gastrointestinal tract (MG7+), 8 thyroid (TGB+), 1 prostate (PSA+), 3 liver (AFP+) and 14 unknown. 3. Immunologic phenotype of 87 lymphomas wereclassified into 66 cases of B-cell, 4 T-cell, 3 hsitocyte, 7 Hodgkin' s diseases and 7 unclear. The above results suggest that immunohistochemlcal method may be used as a new method of diagnosing and differentiating epithelial and non-epithelial tumors, detecting primary focus of metastatic cncer, differentiating between reactive proliferation adn lymphome and specifying immunologic phenotype of lymphoma in cell smears of fine- needle aspiration.This paper reports 25 kinds of polyclonal or monoclonal antibodies by ABC immunohistochemical technique used for 253 cell smears by fine-needle aspiration. The results were,1. Immunohistochemical diagnosis were classified into 136 metastatic cancers ( K12+ EMA+ CEA+ LCA-),92 lymphomas (LCA+ k12- EMA- CEA-), 4 mesenchymal tumors (Vimentin+), 3 melanomas (S-100+NSE+). 15 reactive proliferations (k+λ4+ CD+ CD8+) and 3 unspecified.2. The origin of 70 metastatic cancers were classified into 36 lung (HLC3-AB+), 4 gastrointestinal tract (MG7+), 8 thyroid (TGB+), 1 prostate (PSA+), 3 liver (AFP+) and 14 unknown. 3. Immunologic phenotype of 87 lymphomas wereclassified into 66 cases of B-cell, 4 T-cell, 3 hsitocyte, 7 Hodgkin' s diseases and 7 unclear. The above results suggest that immunohistochemlcal method may be used as a new method of diagnosing and differentiating epithelial and non-epithelial tumors, detecting primary focus of metastatic cncer, differentiating between reactive proliferation adn lymphome and specifying immunologic phenotype of lymphoma in cell smears of fine- needle aspiration.

关 键 词：Immunohistochemlstry CYTOLOGY Antibody. 

分 类 号：R73[医药卫生—肿瘤]