1—及3—硝基苯并[a]芘混合物在大鼠肝微粒体中代谢产物的探讨  

Metabolism of the 1-and 3-Nitrobenzo [a]pyrene by Rat Liver Microsomes

作　　者：陈小薇[1] 肖颖[2] 

机构地区：[1]广州医学院化学致癌研究所 [2]中国预防医学科学院营养与食品卫生研究所

出　　处：《广州医学院学报》1993年第3期20-23,共4页Academic Journal of Guangzhou Medical College

摘　　要：本文分析了1—硝基苯并[a]芘和3—硝基苯并[a]芘混合物在体外有氧条件下的代谢产物及代谢速率,并同单一的1—硝基苯并[a]芘和3—硝基苯并[a]芘的代谢产物进行比较。结果表明,1—及3—硝基苯并[a]芘混合物的代谢产物与单一的1—或3—硝基苯并[a]芘的代谢产物是相同的;而且两者按摩尔比等量混合后的代谢速率也是一致的。实验还表明,需要把高压液相色谱仪的反相柱和正相柱结合起来使用,才能把1—和3—硝基苯并[a]芘混合物的代谢产物完全分开。Metabolism of the 1—and 3—nitro—BaP mixture (in an equal ratio) by liver microsomes of rats pretreated with 3—methyicholanthrene is described in this paper. The incubation products were first separated by reversed—phase HPLC, then by normal—phase HPLC. Upon comparison of HPLC retention times and UV—visible absorption spectra of the metabolites with those of the authentic standards, 1—nitro—BaP trans—7,8—dihydrodiol, 1—nitro—BaP trans—9,10—dihydrodiol, 3—nitro—BaP trans—7,8—dihydrodiol, and 3—nitro—BaP trans—9, 10—dihydrodiol were identified. Upon determining the HPLC peak area are comparing the UV—visible molar extinction coefficient at 254 nm, the metabolism rate between 1—and 3—nitro—BaP was found to be similar. These result indicate that: 1. metabolism of the 1—and 3—nitro—BaP mixture yields similar metabolism pattern as those obtained from the metabolism of 1—nitro-BaP or 3—nitro—BaP; 2. the metabolism rate between 1—and 3—nitro-BaP is similar; 3. it requires both the reversed—phase and normal-phase HPLC for complete separation of the isomeric 1—and 3—nitro—BaP trans—dihydrodiol metabolites. Since all these trans—dihydrodiols have been previously found to be activated metaboites of both 1—and 3—nitro—BaP and since these metabolites are formed from metabolism of the 1—and 3—nitro—BaP mixture, our results suggest that both 1—and 3—nitro—BaP can be hazardous to human health.

关 键 词：1—硝基苯并[a]芘 3—硝基苯并[a]芘 代谢产物 二氢二醇 

分 类 号：R[医药卫生]