孕早期先天性人巨细胞病毒感染的产前诊断  

Prenatal Diagnosis for Congenital Humon Cytomegalovirus Infection Using Nucleic Acid Hybridization Assay.

作　　者：孙永玉[1] 王汉平[1] 

机构地区：[1]同济医科大学附属协和医院妇产科遗传室

出　　处：《中国计划生育学杂志》1993年第4期198-200,255+258,共5页Chinese Journal of Family Planning

摘　　要：应用(32)~P标记人巨细胞病毒(HCMV)Towne株DNA EcoRI B克隆片段作为探针,在国内首次建立了早孕期产前诊断先天性HCMV感染的核酸分子杂交方法。该探针可检测到1.6pg同源DNA序列,与单纯疱疹病毒、EB病毒及人源性细胞DNA无杂交反应。在检测的150份绒毛样本中,10份杂交阳性,阳性率为6.67%。杂交法与病毒分离法相比较,杂交法的敏感性为100%,特异性为71.43%,准确性为86.67%。实验结果表明核酸分子杂交法为早孕期先天性HCMV感染的产前诊断提供了一项有效的手段。A dot blot hybridization assay for the detection of human cytomegalovirus(HCMV) DNA in chorionic villi specimens was first established in our country using nick translated 32P-labelled EcoRl B fragment of HCMV Towne strain DNA as a probe. The probe was shown to be able to detect 1.6pg homologous DNA, and did not cross hybridize with HSV DNA. EBV DNA and human embryo lung(HEL) cell DNA. A total of 150 chorionic villi specimens were detected, and 10 were probe positive. Of 150 specimens, The nucleic acid hybridization assay had a sensitivity of 100%, a specificity of 71% and an accuracy of 86% when compared with conventional virus isolation(CVI). The results suggested that nucleic acid hybridization assay was more sensitive but less specific than CVI method, and proved to be useful for prenatal diagnosis of congenital HCMV infection.

关 键 词：巨细胞病毒 先天性感染 核酸分子杂交 产前诊断 

分 类 号：R169[医药卫生—公共卫生与预防医学]