细菌L型败血症快速诊断新方法的临床研究  

A NEW METHOD FOR RAPID DIAGNOSIS OF CEPTICEMIA INFECTED WITH L FORM OF BACTERIA

作　　者：杨光裕[1] 金兰[1] 李维周 刘玉华[1] 张同玉 杨平[1] 陈文鹏[1] 桂红[1] 

机构地区：[1]河南省新乡市中心医院,新乡453000

出　　处：《河南医学研究》1993年第2期174-176,共3页Henan Medical Research

摘　　要：细菌 L 型败血症的诊断方法,目前国内外均采用患者血进行 L 型培养同,需时7～14天。1985年我们采取患者末梢血涂片,直接用光学显微镜在红细胞上查菌,并用血培养进行对照,经临床211例对照结果证实快速诊断准确度为95.7%,可在两小时内报告结果。为了提高分辨率和准确性,改进了革兰氏染色法,建立了快速诊断的检验程序。1988年6月至1991年10月为486例患者及时明确诊断,使败血症治疗的有效率由80%提高到97.5%。为了进一步提高快速诊断的特异性,我们应用酶联免疫 PAP 法快速诊断金黄色葡萄球菌 L 型败血症,用葡萄球菌兔免疫血清,与患者进行酶联免疫反应,同时作光镜观察及细菌培养共30例,结果光镜、酶免及细菌培养均为26例阳性,三者符合率100%。In 1985,We Found that the L form of bacteria aften adhere to or enter erythrocyte.Accrding to this finding of our study,the rapid diagnosis can be made by peripheral blood smear examination with light microscope for cepticemia infected with L form of bacteria.From June 1988 to October 1991,211 patients were selevted to take the examination.The blood culture or these patients was also made as control.The result showed that the accuracy for this new method was 95.7%.The enzyme immunoassay(PAP Method)was also made to 30 patients to have confirmed the accuracy of the new examination for staphylococcus aureus cepticemia.The improved Grams stain and operating procedure have also been established.The authors believe that the new method for rapid diagnosis of cepticemia infected with L form of bacteria is fast,accurate and reliable.

关 键 词：细菌L型败血症的速诊法 

分 类 号：R[医药卫生]