CPAI分光光度法测定血清中微量镁  

Spectrophotometric determination of trace magnesium in serum with chlorophosphomarto I(CPAI)

作　　者：何亚楠[1] 

机构地区：[1]江苏镇江医学院

出　　处：《右江民族医学院学报》1993年第3期202-204,共3页Journal of Youjiang Medical University for Nationalities

摘　　要：在pH9.50～11.50硼砂缓冲溶液中,血清中微量镁与偶氮氯膦I(CPAI)在表面活性剂Tween-20存在下形成稳定络合物,该络合物最大吸收波长为576nm,摩尔吸光系数为6.56×10~4L/mol·Cm。镁浓度在0～15.5μg/25ml范围内符合比耳定律。本法用于血清中镁的测定,结果满意。In the sodium tetraborate buffer system ( pH9.50～11.50), the trace mag- nesium in serum combined with CPAI in the presence of Tween-20, forming a stable complex. The maximum absorption wavelength and molar absorptivity of the complex was 576 nm and 6.56 × 10~4L/mol.cm respectively. Beer's Law was obeyed in the concentration range of Mg 0～15.5 μg/ 25ml. The method has been applied to the determination of trace magnesium in serum with satis- factory result.

关 键 词：血清 镁 分光光度测定法 微量元素 

分 类 号：R[医药卫生]