八肽胆囊收缩素(CCK-8)的合成及其药理评价  

SYNTHESIS-AND PHARMACOLOGICAL APPRAISE OF CHOLECYSTOKININ OCTAPEPTIDE (CCK-8)

作　　者：凌云[1] 荣扬[1] 陈晓穗[1] 谢邦铁[1] 胡晓愚 文永均[2] 

机构地区：[1]中国人民解放军海军总医院中心实验科,北京100037 [2]兰州大学生物系,兰州730000

出　　处：《中国药物化学杂志》1993年第1期39-41,46,共4页Chinese Journal of Medicinal Chemistry

摘　　要：本文用Merrifield固相合成法合成八肽胆囊收缩紊(CCK-8)。酪氨酸酚羟基硫酯化反应用吡啶-三氧化硫络合物进行,快原子轰击质谱和氨基酸组分分析结果与理论值一致。在离体豚鼠胆囊收缩活性实验中,证实了CCK-8的缩胆囊作用。Synthesis of cholecystokinin octapeptide (CCK-8) by solid phase peptide synthesis using MBHA resin and Boc stretagy. The phenolic hydroxyl of Tyr residue in CCK-8 was sulfated by pyridine-sulfur trioxide complex. CCK-8 was characterizied by FAB-MS and amino acid analysis. CCK-8 was assayed for its cholecystokinetic activity with guinea gallbladders in vitro. It was proved that this synthetic CCK-8 had a cholecystokinetic activity.

关 键 词：八肽胆囊收缩素 多肽合成 吡啶-三氧化硫络合物 离体胆囊 

分 类 号：R914[医药卫生—药物化学]