应用市售抗HCVELISA试剂检测血清抗HCV·IgM的研究  

A New Assay for Detection of Anti-HCV IgM in Sera of Patients with Hepatitis

作　　者：李方和[1] 郭林生[1] 喻植群[1] 郝连杰[1] 

机构地区：[1]同济医科大学附属同济医院临床免疫研究室

出　　处：《华中科技大学学报（医学版）》1993年第S1期61-64,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：采用市售抗HCVELISA试剂盒,葡萄球菌A蛋白(SPA)菌体及酶标记抗人IgM建立血清抗HCV·IgM 检测 SPAELISA。以多种实验对其方法的特异性进行了考核。将其用于临床检测,急肝患者抗 HCV·IgM 检出率明显高于抗 HCV·IgG(11/16,8/16,P<0.05),输血相关性肝炎、散发性肝炎及其高频度社交人群两者的检出率分别为 42.86％与 78.57％,27.27％与 36.36％,及 0与8.47％。且急肝患者抗 HCV·IgM 阳性率显著地高于慢性肝炎患者。A new assay for detection of specific TgM antibody against HCV (anti-HCV IgM) in serum has been developed using the second generation anti-HCV ELISA kit (specific for anti-HCV IgG) .Straptococus protein A bearing germs and peroxidase labelled goat anti human IgM(HRP-GAHM). The specificity of this new method was proved by multiple sub stitution tests,block tests,and a distroying test with β-mecaptoethanol (2-ME). The detection rates of anti-HCV IgM and anti-HCV IgG were 68. 75% and 50% in acute hepatitis pa tients ,42. 86% and 78. 57% in transfusion related hepatitisones,27. 27% and 36. 36% in sporadic hepatitis ones,and zero and 8. 47% in the population with active social activities, respectivley. On the other hand, the positive rate of anti HCV IgM in acute hepatitis (68. 75%) was higher than in chronic hepatitis (27. 08%).

关 键 词：肝炎 病毒性 人 肝炎抗体 免疫球蛋白类 μ链 酶连接免疫吸附测定 

分 类 号：R[医药卫生]