口腔厌氧螺旋体培养条件的研究  

Study of Culture Conditions for Oral Spirochetes

作　　者：杨霖[1] 李成章[1] 白瑞珍[1] 汤纪路[1] 

机构地区：[1]湖北医学院微生物学教研室,武汉430071

出　　处：《中国微生态学杂志》1991年第4期78-81,共4页Chinese Journal of Microecology

摘　　要：口腔厌氧螺旋体的分离培养在国内尚属还未解决的问题,本研究对培养口腔厌氧螺旋体的培养基,培养条件及与梭形杆菌的共生关系进行了探索,制备出适宜于口腔各类厌氧螺旋体生长的“螺旋体大豆消化液选择培养基”(简称SSSM),使用本培养基从40例青少年牙周炎患者牙周袋分泌物标本分离出小齿密螺旋体19株,大齿密螺旋体4株,疏螺旋体4株,另分离出梭形杆菌21株,其结果显示出患者牙周袋及健康人龈沟标本中厌氧螺旋体及梭形杆菌培养阳性率二者之间有高度显著性差异(P<0.001),进一步揭示出牙周炎与口腔厌氧螺旋体之间关系密切。并对分离出的菌株做了常用抗菌素的药敏试验。In this paper medium and culture conditions for oral spirochetes were searched. 19 strain Treponema microdentium, 4 strain Treponema macrodentium and 4 strain Borrelia were isolated from 40 juvenile periodontitis in 'spirochetes soy selective agar'. 21 strain Fusobacterium also were isolated from the same samples. The results indicate relation between oral spirochetes and periodontitis and symbiotic relation between oral spirochetes and fusobacterium. And sensitivity of medicines to spirochetes isolated were done.

关 键 词：厌氧螺旋体 梭形杆菌 SSSM 

分 类 号：Q[生物学] R[医药卫生]