何杰金氏病的免疫组织化学研究  

THE IMMUNOHISTOCHEMISTRY ASSAY OF HODGKIN'S DISEASE

作　　者：王晓彦[1] 董英春[1] 托娅[1] 

机构地区：[1]内蒙古医学院病理解剖学教研室

出　　处：《内蒙古医科大学学报》1991年第1期24-28,共5页Journal of Inner Mongolia Medical University

摘　　要：对71例福尔马林固定、石蜡包埋的活检淋巴组织用ABC免疫组织化学法进行12种凝集素,LeuM_1,α_1-AT,α_1-ACT及Lys标记,发现PSA,ConA,LCA,RCA,PHA,PNA,α_1-AT,α_1-ACT及Lys对HD肿瘤细胞及H-M胞质阳性,并且两者胞质反应形式一致。这6种凝集素在H-M胞质可形成GB,形成GB的H-M称为G细胞。凝集素及α_1-AT,α_1-ACT及Lys标记结果支持HD瘤细胞来源于H-M.LeuM_1及PNA只对HD瘤细胞阳性,并标记少数H-M,对其余成分阴性,故LeuM_1及PNA对HD的诊断、鉴别诊断有意义。HD恶性程度越高,则G细胞越多。71 cases of formalin-fixed and paraffin—embedded lymphoid tissue specimens were examined with 12 kainds of lectins and LeuM_1, α_1—antitrypsin (α_1-AT), α_1-antichymotrypsin (α_1—ACT), lysozyme (Lys) labelling by means of ABC immunohistochemical technique. The cytoplasmic diffusion and globular binding (GB) patterns of histiocyte-macrophage (H-M) and Reed-Sternberg (R-S) cells were noted with 6 kinds ofletins (PSA, ConA, LCA, RCA, PHA, PNA) and α_1-AT, α_1-ACT, Lys labelling, and the cytoplasmic reaction were similar in H-M and R—S cells. Thus, R—S cells may be derived from H—M. The H—M containing GB was called G—cells. Large numbers of G—cells were present in most HD cases of the lymphocyte depleted type (LD) and the mixed cellular type (MC), Therefore, G-celis could be an abnormal variant of H—M and the higher degree of malignancy the more of G cells in number. H—M rarely reacted with LeuM_1 fibroblasts, plasma cells and lymphocytes were negative with LeuM_1and PNA staining. Though H—M somtimes reacted with PNA, R-S cells and H—M could be clearly distinguished under the microscope. NHL was negative with PNA staining. It is indicated that LeuM_1and PNA staining are valuable to the diagnosis and differential diagnosis of HD.

关 键 词：何杰金氏病 免疫组织化学 凝集素 组织细胞-巨噬细胞 

分 类 号：R[医药卫生]