Comparison of the Two Chromosome Forms of Paragonimus Westermani Using DNA Restriction Fragment Size and DNA Hybridization  

作　　者：王恩荣 郑韧坚 george D.Cain 

机构地区：[1]Department of Parasitology, China Medical University. China [2]Shanghai Institute of Animal Parasitology [3]Department of Biology, The University of Iowa, IA, USA

出　　处：《中国医科大学学报》1991年第S2期24-28,共5页Journal of China Medical University

摘　　要：Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurther evidence that the distribution of restriction enzyme sites (with 3enzymes) on repeated DNA of both was similar. But with Pst Ⅰ、Dde Ⅰ、Hae Ⅲand Hpa Ⅱ. They revealed polymorphism which also occured individually ineach form tested separatedly with hybridization technique. Mutation of recog-nize sites by enzymes, which is resulted from the alternation of gene sequance,has occured during evolution. Additionally, it is probably possible, based on PstⅠand Dde Ⅰdigest pattern to distinguish between diploid and triploid Parag-onimus westermani and group the Paragonimus simply and rapidly.Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurther evidence that the distribution of restriction enzyme sites (with 3enzymes) on repeated DNA of both was similar. But with Pst Ⅰ、Dde Ⅰ、Hae Ⅲand Hpa Ⅱ. They revealed polymorphism which also occured individually ineach form tested separatedly with hybridization technique. Mutation of recog-nize sites by enzymes, which is resulted from the alternation of gene sequance,has occured during evolution. Additionally, it is probably possible, based on PstⅠ and Dde Ⅰ digest pattern to distinguish between diploid and triploid Parag-onimus westermani and group the Paragonimus simply and rapidly.

关 键 词：PARAGONIMUS Westermani CHROMOSOME form DNA HYBRIDIZATION DNA RESTRICTION FRAGMENT 

分 类 号：R[医药卫生]