DNA CYTOPHOTOMETRIC MEASUREMENTS OF CANINE STOMACH CARCINOGENESIS INDUCED BY N-ETHYL-N'-NITRO-N-NITROSO GUANIDINE  

作　　者：朱人敏 萧树东 江绍基 王瑞年 刘文忠 胡运彪 

机构地区：[1]Shanghai Institute of Digestive Disease, SSMU, Shanghai

出　　处：《Medical Bulletin of Shanghai Jiaotong University》1990年第1期95-100,共6页上海交通大学学报（医学英文版）

摘　　要：Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-ethyl-N’-nitro-N-nitrosoguanidine (ENNG). The mean values and standard deviations of nuclear DNA and area in different groups of lesions were as follows: normal gastric mucosal 0.03±2.30 AU and 28.76±5.85 μm^2; atrophic gastritis 12.04±3.34 AU and 28.69±8.02/μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58±10.74 μm^2; severe dysplasia 24.01±4.48 AU and 56.64±21.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant difference statistically between normal gastric mucosa and gastric carcinoma (P【0.01). The nuclear area of gastric carcinoma increased with DNA content (r=0.73, P【0.01), and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in carcinoma. These findings indicate that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.Measurement of DNA content and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG). The mean values and standard deviations of nuclear DNA and area in different groups of lesions were as follows: normal gastric mucosal 0.03±2.30 AU and 28.76±5.85 μm^2; atrophic gastritis 12.04±3.34 AU and 28.69±8.02/μm^2; mild dysplasia 13.52±3.73 AU and 28.23±8.12μm^2; moderate dysplasia 20.88±4.57 AU and 47.58±10.74 μm^2; severe dysplasia 24.01±4.48 AU and 56.64±21.53μm^2; well-differentiated adenocarcinoma 33.07±9.38 AU and 72.99±15.57μm^2, respectively. The nuclear DNA content and nuclear area showed a significant difference statistically between normal gastric mucosa and gastric carcinoma (P<0.01). The nuclear area of gastric carcinoma increased with DNA content (r=0.73, P<0.01), and the distribution patterns of DNA content in the histogram showed that diploidy was decreased and polyploidy was increased in carcinoma. These findings indicate that DNA ploidy patterns and nuclear area could be a useful index in differentiated carcinoma and precancerous lesions.

关 键 词：DNA content MICROSPECTROPHOTOMETRY PLOIDY experimental gastric carcinoma ENNG 

分 类 号：R[医药卫生]