传染性牛鼻气管炎病毒单克隆抗体的制备及其应用  

Preparation and Application of Monoclonal Antibodies of Infections Bovine Rhinofracheitis Virus(BHV-l)

作　　者：李健强[1] DavidT.Shen D.Burger J.R.Gorham W.C.Davis 

机构地区：[1]西北农业大学兽医系 [2]美国华盛顿州立大学

出　　处：《西北农林科技大学学报（自然科学版）》1990年第2期33-41,共9页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：采用改进的融合技术得到318个杂交瘤细胞系。经ELISA筛选,这些细胞系所分泌的抗体均和病毒抗原发生反应。亚克隆出26个杂交瘤细胞系,制出特异的单克隆抗体,并采用ELISA、放射免疫沉淀试验(RIPA)、SDS-PAGE、免疫印迹及病毒中和试验等对单克隆抗体的特性作了鉴定。应用单克隆抗体对牛疮瘆病毒Ⅰ型Bovine HerpesVirus I(BHV—Ⅰ)主要结构蛋白作了分析。318 hybridoma cell lines have been obtained by using improved fusion method. These cell lines screened by ELISA are the secreted specific antibodies reacting to BHV-1 virus antigen. 26 hybridoma cell lines were cloned to produce specific monoclonal antibodies to the virus antigen. The characteristics of monoclonal antibodies were identified with ELISA, RlPA. SDS-PAGE, Immunoblot and Virus Neutralization test. The major BHV-1 structural peptides were analysed by monoclonal antibodies.

关 键 词：传染性牛鼻气管炎病毒 单克隆抗体 SDS-PAGE 免疫印迹 病毒中和试验 

分 类 号：S[农业科学]