应用戊二醛对百日咳抗原进行解毒的研究  

Investigation on the Detoxification of the Pertussis Protective Antigens with Glutaldhyde

作　　者：刘德铮[1] 肖詹蓉[1] 李万臣[1] 王兴宇 赵宝明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1990年第4期181-184,共4页Chinese Journal of Biologicals

摘　　要：本文报道应用戊二醛对百日咳保护性抗原解毒,并研究解毒条件与毒性及免疫原性的关系。在百日咳保护性抗原或无细胞百日咳菌苗的制造中,应用0.05%戊二醛(GA)于20℃作用4～6小时,再加入0.15～0.2%赖氨酸继续作用2小时,可以得到最佳解毒效果。用于制造无细胞百日咳菌苗符合菌苗检定要求,经37℃存放1个月后未见毒性逆转现象发生。The several methods of the detoxification on the protective antigens of B.pertussis were studied.It was found the relationships between the depth of deto- xification and the toxicity and immunogenicity of antigens.The toxicity of the anti- gens could not be detoxified completely in presence of more than 0.02% lysine before glutaldhyde was added.The temperature on detoxification was highly important.In 20℃,the effect of detoxification was complete but not in 4℃.The best procedure of detoxification for the antigens of B.pertussis was that the antigens were treated with glutaldhyde for 4～6 h at 20℃,then 0.15～0.2% lysine was added and reacted aga- in for 2 h at the same temperature.By using this procedure,the toxicity reversion of the acellular pertussis vaccine has not been found after kept under 37℃ for 1 month.

关 键 词：戊二醛 百日咳抗原 

分 类 号：R977[医药卫生—药品]