Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acids  

作　　者：Qi Deyao and Zhuang Yunlong (Department of Chemistry and Chemical Engineering, Shanghai University of Technology, Shanghai)Chi Qijin (Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun) 

出　　处：《Chemical Research in Chinese Universities》1990年第4期295-299,共5页高等学校化学研究（英文版）

摘　　要：Two tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operating conditions and properties with respect to buffer sort, pH of the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, such as Km and Km, of L-glutamine deaminasc and L-as-paragine deamlnase have been obtained by means of these tissue electrodes.Two tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operating conditions and properties with respect to buffer sort, pH of the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, such as Km and Km, of L-glutamine deaminasc and L-as-paragine deamlnase have been obtained by means of these tissue electrodes.

关 键 词：Biosensors Tissue electrodes BIOCATALYSIS APPLE CACTUS 

分 类 号：O6[理学—化学]