白鲜碱对白色念珠菌体外抑制作用初探  

In vitro antifungal activity of dictamnine against candida albicans

作　　者：施琳俊[1,2] 薛婷君[3] 吴岚[1,2] 朱彩莲[2] 周曾同[1,2] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院口腔黏膜科,上海200011 [2]上海市口腔医学重点实验室,上海200011 [3]同济大学附属东方医院口腔科,上海200120

出　　处：《临床口腔医学杂志》2011年第11期654-657,共4页Journal of Clinical Stomatology

基　　金：上海市卫生局中医药科研基金(2006L031A);上海市自然科学基金(11ZR1420400);高等学校博士学科点专项科研基金

摘　　要：目的:检测白鲜碱对白色念珠菌的抑制作用,及其对白色念珠菌细胞周期的影响。方法:采用白色念珠菌标准菌株(ATCC76615)为研究对象,用经典方法测定白鲜碱对白色念珠菌的最小抑菌浓度(MIC),抑制生物膜50%(SMIC50)的药物浓度,流式细胞仪分析白鲜碱对白色念珠菌细胞周期的影响。结果:白鲜碱对白色念珠菌的MIC为312.5μg/mL,对生物膜的SMIC50为1250μg/mL,白鲜碱能使白色念珠菌生长停滞。结论:白鲜碱具有抑制白色念珠菌生长的能力。Objective：To investigate the in vitro antifungal activity of dictamnine against candida albicans and the influence of dictamnine on the cell cycle of candida albicans.Method：NCCLS M27-A2 broth microdilution method was accessed to evaluate the in vitro activity of dictamnine against candida albicans（ATCC 76615）.XTT-reduction method was used to test the SMIC50 of dictamnine against candida albicans biofilms.Flow cytometer was applied to determine the effect of dictamnine on the cell cycle of candida albicans.Result：MIC of dictamnine against candida albicans was 312.5 μg/mL.SMIC50 of dictamnine against candida albicans biofilms was 1250 μg/mL.Dictamnine can stop the growth cycle of Candida albicans.Conclusion：Dictamnine displayed in vitro antifungal activity against candida albicans.

关 键 词：白色念珠菌 生物膜 白鲜碱 流式细胞仪 细胞周期 

分 类 号：R781.5[医药卫生—口腔医学]