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作 者:孙笑笑[1] 王科[1] 冯红蕾[1] 罗进勇[1] 王虹[1] 张彦[1]
机构地区:[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016
出 处:《中国生物制品学杂志》2011年第11期1285-1289,共5页Chinese Journal of Biologicals
基 金:国家自然科学基金(30800658)
摘 要:目的探讨DN-ALK2对人乳腺癌细胞MDA-MB-231增殖、迁移和侵袭能力的影响。方法 RT-PCR法检测MDA-MB-231细胞中ALK2基因mRNA的转录;分别以腺病毒DN-ALK2和RFP感染MDA-MB-231细胞,并设空白对照组,通过MTT法、平板集落形成试验、细胞划痕试验及Transwell侵袭试验检测细胞的增殖、集落形成、迁移及侵袭能力。结果 MDA-MB-231细胞中内源性表达ALK2。腺病毒DN-ALK2和RFP感染MDA-MB-231细胞36 h后,荧光表达量一致,约为70%。MDA-MB-231/DN-ALK2细胞中高表达DN-ALK2。与MDA-MB-231/RFP组相比,MDA-MB-231/DN-ALK2组细胞的增殖活力、集落形成率及划痕愈合率均显著降低(P<0.05),穿膜细胞数也明显减少(P<0.05);而MDA-MB-231/RFP组与空白对照组比较,差异无统计学意义(P>0.05)。结论DN-ALK2可以在体外抑制乳腺癌MDA-MB-231细胞的增殖、迁移与侵袭。Objective To investigate the effect of DN-ALK2 on proliferation, migration and invasion abilities of human breast cancer MDA-MB-231 cells. Methods The transcription of ALK2 mRNA in MDA-MB-231 cells was determined by RT-PCR. MDAMB-231 cells were infected with adenovirus DN-ALK2 and RFP respectively, then determined for proliferation level by M'Iq" method, for colony formation by plate colony formation test, for migration ability by cell wounding test, and for invasion ability by Transwell invasion test, using those uninfected as blank control. Results ALK2 was highly expressed in MDA-MB-231 cells. Both the expression level of RFP in MDA-MB-231 cells 36 h after transfection with DNA-ALK2 and RFP were about 70%. DN-ALK2 was highly expressed in MDA-MB-231 / DN-ALK2 cells. The proliferation activity, colony formation rate, healing rate of wound and count of cells crossing the matrix barrier were significantly lower in MDA-MB-231/DNA-ALK2 group than in MDA-MB-231/RFP group (P 〈 0. 05), while showed no significant difference in MDA-MB-231/RFP and blank control groups (P 〉 O. 05). Conclusion DN-ALK2 inhibited the proliferation, migration and invasion of MDA-MB-231 cells in vitro.
关 键 词:DN.ALK2腺病毒 乳腺肿瘤 细胞增殖 细胞运动 肿瘤浸润
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