乌司他丁和泰索帝对人乳腺癌细胞MDA-MB-231中uPA、uPAR、ERK表达的影响  

Effects of Ulinastatin and Taxotere on Expressions of Urokinase Type Plasminogen Activator(uPA),uPA Receptor(uPAR) and Extracellular Signal-regulated Kinase in Human Breast Cancer MDA-MB-231 Cells

作　　者：罗杰[1] 高峰[1] 赵晓亮[1] 孙治君[1] 

机构地区：[1]重庆医科大学第二附属医院普外科,重庆400016

出　　处：《中国生物制品学杂志》2011年第11期1329-1332,共4页Chinese Journal of Biologicals

基　　金：重庆市科委基金项目(CSCT;2008AC5082)

摘　　要：目的探讨乌司他丁(Ulinastatin,UTI)和泰索帝(Taxotere,TXT)对体外培养的人乳腺癌细胞MDA-MB-231中u-PA、uPAR、ERK表达的影响。方法将MDA-MB-231(ER-)细胞分为4组:UTI组(UTI 800 U/ml)、TXT组(TXT 3.7μg/ml)、UTI+TXT组(UTI 800 U/ml+TXT 3.7μg/ml)、对照组(等量生理盐水)。给药后24 h,分别采用荧光定量RT-PCR检测各组细胞中uPA、uPAR、ERK基因mRNA的水平,Western blot法检测各组细胞中uPA、uPAR、p-ERK1/2蛋白的表达水平。结果 UTI组和UTI+TXT组MDA-MB-231(ER-)细胞中uPA和uPAR基因mRNA的水平均明显低于对照组(P<0.05),而TXT组中二者的表达水平均明显高于对照组(P<0.01),各组间ERK基因mRNA的水平差异无统计学意义(P>0.05);UTI组和UTI+TXT组中uPA、uPAR和p-ERK1/2蛋白的表达水平均明显低于对照组(P<0.01),而TXT组中3种蛋白的表达水平均明显高于对照组(P<0.05)。结论UTI可抑制MDA-MB-231细胞中uPA、uPAR、p-ERK的表达,而TXT可上调三者的表达。Objective To investigate the effects ulinastatin （UTI） and Taxotere （TXT） on expressions of urokinase plasminogen activator （uPA）, uPA receptor （uPAR） and extracellular signal-regulated kinase （ERK） in human breast cancer MDA-MB-231 cells. Methods MDA-MB-231 （ER-） cells were divided into UTI, TXT, UTI ＋ TXT and control groups, and treated with 800 U/ml UTI, 3.7 p.g/ml TXT, 800 U/ml UT1 ＋ 3.7 μg/mI TXT and an equal volume of physiological saline respectively. The uPA, uPAR and ERK mRNA levels in cells of various groups 24 h after treatment were determined by RT-PCR, while the expression levels of uPA, uPAR and p-ERK1 /2 proteins by Western blot. Results The uPA and uPAR mRNA levels were significantly lower in UTi and UTI ＋ TXT groups （P 〈 0. 05）, while were significantly higher in TXT group than in control groups （P 〈 0. 01）. However, the ERK mRNA levels showed no significant difference in various groups （P 〉 0. 05）. The expression levels of uPA, uPAR and p-ERK1/2 proteins were significantly lower in UTI and UTI ＋ TXT groups （P 〈 0. 01 ）, while were significantly higher in TXT group than in control group （P 〈 0. 05）. Conclusion UTI inhibited, while TXT up-regulated the expressions of uPA, uPAR and p-ERK in MDA- MB-231 ceils.

关 键 词：乌司他丁 泰索帝 乳腺肿瘤 尿激酶型纤溶酶原激活物 尿激酶型纤溶酶原激活物受体 细胞外信号调节激酶 

分 类 号：R737.9[医药卫生—肿瘤] R977.3[医药卫生—临床医学]