准分子激光原位角膜磨镶术中负压吸引兔眼视网膜基因谱的表达变化  

Study on gene expression profiles of rabbit retina transient lOP during

作　　者：赵海霞[1] 牛春梅[1] 关文英[1] 

机构地区：[1]内蒙古医学院附属医院眼科,010050

出　　处：《中华眼科杂志》2011年第11期1019-1024,共6页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金（30660194）;内蒙古自然科学基金;内蒙古医学院附属医院重大课题

摘　　要：目的应用基因芯片技术研究负压吸引后兔视网膜的基因表达谱，探讨负压吸引引起RGCs凋亡可能的分子学机制。方法12只健康新西兰大耳白兔随机分为实验对照组和负压吸引3min组。负压吸引3min组分别于术后即刻（0d）、7d和10d处死，取视网膜组织提取总RNA，利用Agi1ent公司兔单标芯片检测视网膜组织的基因表达谱，并分析差异表达基因。结果负压吸引3rain术后即刻差异表达基因共704条。上调基因485条，下调基因219条，其中凋亡相关基因共筛选出6条，上调5条，下调1条，上调表达较高的基因是CRYAA、CRYAB和TLR3，下调表达较高的基因是KRT18。负压吸引3min术后7d差异表达基因共482条。上调基因178条，下调基因304条，其中凋亡相关基因共筛选出4条，上调1条，下调3条，上调表达较高的基因是CRYAB，下调表达较高的基因是IL1—BETA和IL1R1。负压吸引3rain术后10d差异表达基因共402条。上调基因213条，下调基因189条，其中凋亡相关基因共筛选出6条，上调4条，下调2条，上调表达较高的基因是CRYAB、CRYBA3和CRYBB2，下调表达较高的基因是IL1-BETA和IL1R1。在差异表达基因中除凋亡相关基因外还有能量代谢、物质转运、信号传导、发育、解毒抗氧化、免疫反应、细胞运动、细胞组织生物发生、分化和细胞黏附等相关基因。结论负压吸引可使视网膜一些基因表达水平发生变化，其中基因差异倍数较高的基因多数对RGCs凋亡起保护作用。Objective To detect the changes of gene expression profiles of retina by way of gene chip technology, and to investigate possible molecular pathogenesis of RGCs apoptosis caused by transient high IOP. Methods Twenty-two new Zealand white rabbits were randomized to experimental control and negative pressure suction 3 min groups. The rabbit were executed at different time points of post-LASIK, such as postoperative instant,7 d, 10 d,then the total RNA of retinal tissue was extracted to detect gene expression profiles by the way of Agilent rabbit one-way gene chip. Results Instant after negative pressure suction, the genes differential expressed had 704 genes. Of these, the expression of 485 genes were unregulated and 219 genes down regulated. The higher expression of apoptosis genes were CRYAA, CRYAB,TLR3 and KRT18. 7 d after negative pressure suction, the genes differential expressed had 482 genes. Of these, the expression of 178 genes were unregulated and 304 genes down regulated. The higher expression of apoptosis genes were CRYAB, ILl-BETA and IL1R1. 10 d after negative pressure suction, the genes differential expressed had 402 genes. Of these, the expression of 213 genes were unregulated and 189 genes down regulated. The higher expression of apoptosis genes were CRYAB, CRYBA3, CRYBB2, ILl-BETA and IL1 R1. Conclusion The gene expression had changed after negative pressure suction. The genes with higher fold change protected RGCs from apoptosis.

关 键 词：准分子激光原位角膜磨镶术 眼压 基因芯片 

分 类 号：R779.63[医药卫生—眼科]