2-脱氧-D-葡萄糖与奥沙利铂对人肝癌SMMC-7721细胞增殖及凋亡的影响  

Effects of 2-deoxy-D-glucose and oxaliplatin on proliferation and apoptosis of human hepatoma cell line SMMC-7721

作　　者：耿翔[1] 周奇[2] 刘超[1] 尚闯[1] 庞志刚[1] 

机构地区：[1]郑州大学第二附属医院普外科,河南郑州450003 [2]中山大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国病理生理杂志》2011年第11期2101-2105,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.S2011010004247)

摘　　要：目的:研究糖酵解抑制剂2-脱氧-D-葡萄糖(2-deoxy-D-glucose,2-DG)与奥沙利铂(ox-aliplatin,L-OHP)单独及联合应用对人肝癌细胞SMMC-7721增殖及凋亡的影响。方法:将2-DG及L-OHP单药及联合用药作用于人肝癌细胞SMMC-7721,MTT法检测各组细胞的生长抑制率,并计算两药的协同作用q值,流式细胞仪检测各组细胞周期及凋亡情况,caspase-3试剂盒检测caspase-3活性。结果:不同浓度的2-DG和L-OHP均可使肝癌细胞增殖受到抑制,并呈时间、浓度依赖性,两药合用后对细胞的增殖抑制作用明显增强(P<0.05)。2-DG可诱导细胞凋亡,使细胞周期阻滞于G2/M期;与L-OHP合用后细胞凋亡率更高,可阻滞细胞于G2/M期和S期。Caspase-3活性在两药联合应用时明显增强。结论:2-DG能有效抑制SMMC-7721细胞生长增殖,并诱导其凋亡;当其与L-OHP联合应用时能增强L-OHP的抗肿瘤作用,其机制可能与增加caspase-3活性有关。AIM： To investigate the effects of 2-deoxy-D-glucose（2-DG） or oxaliplatin（L-OHP） alone and the combination of both on the proliferation and apoptosis of hepatoma cell line SMMC-7721 in vitro. METHODS： Human hepatoma cell line SMMC-7721 was treated with 2-DG or L-OHP alone,or both.The inhibitory effect on the proliferation of SMMC-7721 cells was estimated by MTT method.The q value,which represents synergistic effect,was determined.Apoptotic rate and cell cycle were assayed by flow cytometry.The activity of caspase-3 was detected by a caspase-3 activity assay kit. RESULTS： 2-DG or L-OHP at different concentrations inhibited the growth of SMMC-7721 cells obviously and the inhibitory effect on SMMC-7721 cell growth strongly depended on the exposure time and dose.When the 2 drugs worked together,the inhibitory effect was improved（P0.05）.2-DG induced cell apoptosis and arrested the cells at G2/M phase.When combined with L-OHP,the 2 drugs induced more severe apoptosis and arrested the cells at S and G2/M phase.Meanwhile,the activity of caspase-3 increased when the 2 drugs used together. CONCLUSION： 2-DG inhibits the growth of hepatoma cell line SMMC-7721.The combination of 2-DG and L-OHP improves the ability of L-OHP to attack the tumor cells.The mechanism might be related to increasing the activity of caspase-3.

关 键 词：2-脱氧-D-葡萄糖 SMMC-7721细胞 奥沙利铂 瓦博格效应 

分 类 号：R735.7[医药卫生—肿瘤]