小鼠对雨蛙肽诱发急性胰腺炎易感性的品系差异  

Strain difference of susceptibility to caerulein-induced acute pancreatitis in mice

作　　者：张化宇[1,2] 唐寅[1,2] 常青[3] 刘璐[1,2] 王宇辉[1,2] 蒋孟茜[1,2] 韩迎春[1,2] 祁荣[1,2] 刘国庆[1,2] 

机构地区：[1]北京大学心血管研究所,北京100191 [2]北京大学分子心血管学教育部重点实验室,北京100191 [3]北京大学病理学系,北京100191

出　　处：《中国病理生理杂志》2011年第11期2170-2174,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30971241)

摘　　要：目的:小鼠是常用于胰腺炎研究的模式动物。然而不同品系的小鼠似乎对胰腺炎有着不同的易感性,但缺乏系统的对比研究。我们因此比较了C57BL/6J、BALB/c和ICR小鼠对雨蛙肽诱导的急性胰腺炎易感性的差异。方法:2月龄C57BL/6J、BALB/c和ICR雌鼠各12只,随机分为实验组(n=6)和对照组(n=6)。实验组予以腹腔注射雨蛙肽(50μg/kg),每小时1次,共注射7次;对照组同法注射等量生理盐水。分别于第1次注射后的0、3、6、9、12、24 h取小鼠血浆,并于24 h处死小鼠取其胰腺组织。测定各组小鼠的血浆淀粉酶和脂肪酶活性;观察其胰腺组织的病理形态学改变程度及炎症因子表达水平。结果:雨蛙肽诱导胰腺炎后,BALB/c和ICR小鼠的血浆淀粉酶和脂肪酶水平较C57BL/6J小鼠升高更为显著;C57BL/6J小鼠与其它2种品系的小鼠相比,其胰腺病理形态学改变程度较轻,胰腺组织炎症因子表达水平也较低。结论:BALB/c和ICR小鼠对雨蛙肽诱导的急性胰腺炎的敏感性比C57BL/6J小鼠高。这一结果对急性胰腺炎研究中小鼠品系的合理选择具有指导意义。AIM： To study the difference of susceptibility to caerulein-induced acute pancreatitis（AP） among the mice of C57BL/6J,BALB/c and ICR strains.METHODS： Two-month-old female mice of C57BL/6J,BALB/c and ICR strains（12 mice for each strain） were divided into control group（n=6） and experimental group（n=6）,respectively.The mice were intraperitoneally injected with caerulein（50 μg/ kg） in 1 h interval for 7 serial injections in total.The mice in control group were treated with saline according to the same procedure in experimental group.The blood samples were collected at 0 h,3 h,6 h,9 h,12 h and 24 h after the first injection of caerulein or saline for plasma α-amylase and lipase assays.The mice were sacrificed 24 h after AP induction,and the pancreatic tissues were harvested for further investigating the pathological changes and expression of inflammatory factors.RESULTS： After AP induction,the mice of BALB/c and ICR strains demonstrated more dramatic increase in plasma α-amylase activity and lipase activity than those of C57BL/6J mice.C57BL/6J mice showed milder morphological changes and lower expression of inflammatory factors in pancreata than those of BALB/c and ICR mice.CONCLUSION： The mice of C57BL/6J strain have less susceptibility to caerulein-induced AP than that of BALB/c and ICR mice.

关 键 词：小鼠品系 雨蛙肽 急性胰腺炎 易感性 

分 类 号：R576[医药卫生—消化系统]