褪黑素对胃肠运动的影响  

Effects of melatonin on gut motility

作　　者：陈春球[1] 丁卫星[1] 李永渝[2] 

机构地区：[1]同济大学附属第十人民医院胃肠外科,上海200072 [2]同济大学医学院病理生理教研室,上海200092

出　　处：《中国病理生理杂志》2011年第11期2185-2188,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30971168)

摘　　要：目的:研究不同浓度褪黑素(melatonin)对小鼠胃肠运动的影响。方法:雄性C57BL小鼠腹腔内注射1 mg/kg、5 mg/kg、50 mg/kg和75 mg/kg浓度的褪黑素,分别在15 min和45 min后,检测结肠排出玻璃珠的变化;同时,在注射褪黑素75 mg/kg前腹腔内注射褪黑素拮抗剂luzindole(5 mg/kg),研究其对褪黑素作用的影响。另外,通过全胃肠道运输实验(伊文斯蓝排出实验)检测腹腔内注射不同浓度褪黑素(1 mg/kg、5 mg/kg、25 mg/kg和75 mg/kg)时胃肠运动的变化。结果:腹腔内注射褪黑素后15 min,在低剂量组(1 mg/kg)结肠排出玻璃珠的时间缩短,与对照组比较差异显著(P<0.01);在高剂量组(75 mg/kg)结肠排出玻璃珠的时间明显延长(P<0.01),该抑制作用可被褪黑素拮抗剂luzindole有效拮抗(P<0.01)。腹腔内注射褪黑素后45 min,在5 mg/kg剂量时,结肠排出玻璃珠的时间明显缩短(P<0.01);而在1 mg/kg和50 mg/kg时,与对照组比较并无显著差异;在高剂量组(75 mg/kg)结果同上,即结肠排出玻璃珠的时间延长(P<0.05)。在胃肠道运输实验中,腹腔内注射褪黑素1 mg/kg和5 mg/kg后,运输时间缩短,排出伊文斯蓝粪粒所需时间减少,与对照组比较差异显著(均P<0.01);大剂量褪黑素(75 mg/kg)也明显延长胃肠道运输时间(P<0.01)。结论:低剂量褪黑素促进胃肠运动,高剂量褪黑素降低胃肠运动,后者作用可被褪黑素受体拮抗剂luzindole阻断。AIM： To investigate the influence of melatonin at different concentrations on the gut motility.METHODS： Male C57BL mice were used in the study.The mice were intraperitoneally injected with melatonin at doses of 1 mg/kg,5 mg/kg,50 mg/kg and 75 mg/kg.The colon bead expulsive time was measured at 15 min and 45 min after injection.The melatonin antagonist luzindole at a dose of 5 mg/kg was used before injection of 75mg/kg melatonin.The gut transit time was also recorded with oral Evens blue after injection of melatonin.RESULTS： Compared with control group,melatonin at the dose of 1 mg/kg at 15 min decreased the colon bead expulsive time.However,melatonin at high dose（75 mg/kg） prolonged the colon bead expulsive time compared with the control,which was effectively blocked by melatonin antagonist luzindole.Compared with control group,injection of melatonin at the dose of 5 mg/kg at 45 min decreased the colon bead expulsive time.However,melatonin at high dose（75 mg/kg） prolonged the colon bead expulsive time.No difference of colon bead expulsive time between using 1 mg/kg and 50 mg/kg melatonin was observed.In whole gut transit time test,after injection of melatonin at the doses of 1 mg/kg and 5 mg/kg,the time was decreased.The expulsive time of fecal pellet with Evens blue was shorten as compared with control group.However,the gut transit time was significantly prolonged after high dose of melatonin（75 mg/kg） was used.CONCLUSION： Low dose of melatonin increases while high dose decreases the gut motility.Melatonin antagonist luzindole effectively blocks the latter effect.

关 键 词：褪黑激素 胃肠活动 LUZINDOLE 

分 类 号：R363[医药卫生—病理学]