硒对酥梨叶片衰老及抗氧化酶系统的影响  

Effects of Selenium on Leaf Senescence and Antioxidase System in Pyrus bretschneider‘Dangshan Suli’

作　　者：刘群龙[1] 王朵[1] 吴国良[2] 郝国伟[3] 郝燕燕[1] 孙胜[1] 

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]河南农业大学园艺学院,郑州450002 [3]山西省农业科学院果树研究所,山西太谷030815

出　　处：《园艺学报》2011年第11期2059-2066,共8页Acta Horticulturae Sinica

基　　金：国家梨产业体系建设专项(Nycytx-29-30);河南省重大科技攻关项目(092101110600);山西省科技产业化环境建设项目(2006071024);山西省星火计划项目(20090510012)

摘　　要：研究喷施Na2SeO3对砀山酥梨(Pyrus bretschneider Rehd.‘Dangshan Suli’)叶片衰老及抗氧化酶系统的影响。结果表明:与对照相比,喷施低浓度的硒(5mg·L-1),可明显延迟衰老叶片含水量、干样质量、叶绿素和可溶性蛋白含量的下降,显著提高SOD和GSH-Px活性,降低H2O2、和MDA含量,平均延迟落叶7.25d;喷施中等(10mg·L-1)和高浓度(20mg·L-1)的硒对延缓叶片衰老的效果变差,且浓度越高,效果越差;叶面喷硒对POD活性具有抑制作用。硒延缓梨树叶片衰老存在浓度差异,低浓度的硒可能通过提高SOD、GSH-Px等酶的活性参与叶片衰老进程的调控,从而降低膜脂过氧化水平,延长叶片功能期。The effects of selenium（Se）on leaf senescence and antioxidase system in Pyrus bretschneider‘Dangshan Suli’were studied after fruit harvested. The results showed that：Spraying lower Se dosage（5 mg · L-1）delayed the decrease of water content,dry weight per unit area,chlorophyll and soluble protein content significantly,increased the activities of SOD and GSH-Px,decreased the content of H2O2, and MDA significantly in the senescing leaves,thereby deferred leaf senescence about 7.25 days;But the medium（10 mg · L-1）and higher Se dosage（20 mg · L-1）were not so effective in deferring leaf senescence,the higher the dosage,the lower the effect;However,Se inhibited POD activity. Therefore,the effect of Se on leaf senescence varied depending on its dosage,lower Se dosage may be involved in leaf-senescing regulation by enhancing the activities of SOD and GSH-Px,as a result decreases the membrane lipid peroxidation,postpones the functional period of the leaves.

关 键 词：梨 硒 叶片 衰老 抗氧化酶 活性氧代谢 

分 类 号：S661.2[农业科学—果树学]