大黄素抑制体内外胰腺癌转移的实验研究  

Experimental study on anti-metastasis effect of emodin on human pancreatic cancer

作　　者：刘岸 沙丽晓[2] 沈跃[3] 黄莉莉[4] 唐晓[4] 林胜璋[3] 

机构地区：[1]岳阳市第一人民医院,湖南岳阳414000 [2]温州医学院附属第一医院,浙江温州325000 [3]温州医学院附属第二医院,浙江温州325027 [4]温州医学院,浙江温州325000

出　　处：《中国中药杂志》2011年第22期3167-3171,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173606);浙江省自然科学基金项目(Y2080708);浙江省中医药管理局重点项目(2008ZA015,2011ZZ010)

摘　　要：目的:探讨大黄素抑制体内外胰腺癌转移的作用及其机制。方法:不同浓度大黄素(10,20,40μmol.L-1)作用人胰腺癌细胞株SW1990 2 h后,观察大黄素对SW1990细胞迁移和侵袭的作用;不同浓度大黄素(10,20,40μmol.L-1)作用SW1990细胞48 h后,Western blot印迹检测胰腺癌细胞中NF-κB和MMP-9表达的变化;建立胰腺癌原位移植模型,分成对照组、大黄素低、高剂量组(20,40 mg.kg-1),实验结束后观察大黄素对裸鼠胰腺癌转移的抑制作用;免疫组织化学法检测裸鼠胰腺肿瘤组织中CD34,NF-κB,MMP-9的阳性表达。结果:大黄素可抑制体外人胰腺癌SW1990细胞迁移和侵袭,呈浓度依赖性;大黄素可下调人胰腺癌SW1990细胞中NF-κB和MMP-9的表达水平;与对照组相比较,大黄素低、高剂量均可明显抑制瘤荷裸鼠中胰腺癌转移,并下调CD34,NF-κB和MMP-9在胰腺肿瘤组织中的阳性表达(P<0.05)。结论:大黄素可抑制体内外胰腺癌转移,该作用可能通过下调NF-κB和MMP-9表达而实现。Objective： To investigate the anti-metastasis effect of emodin on the pancreatic cancer in vitro and in vivo.Method： Human pancreatic cancer cell line SW1990 was treated with different concentrations of emodin（10,20,40 μmol·L^-1） for 2 h,the effects of emodin on the migration and invasion of SW1990 cells were examined by using wound assay and matrigel counting.Western blot was used to detect the protein expression of NF-κB and MMP-9 in SW1990 cells after various concentrations of emodin（10,20,40 μmol·L^-1） treatment for 48 h.Metastatic model simulating human pancreatic cancer was established by orthotropic implantation of histologically intact human tumor tissue into pancreatic wall of nude mice,and then divided into three groups： control group,low-dose emodin group（L-EMO） and high-dose emodin group（H-EMO）.Eight weeks after implantation,the presences of metastasis were evaluated respectively after the mice were sacrificed.Immunohistochemistry was used to detect the positive expression of CD34,NF-κB and MMP-9 in the tumors.Result： Emodin suppressed the migration and invasion of SW1990 cells in a dose-dependent manner.Western bolt assay indicated that emodin down-regulated the expression of NF-κB and MMP-9 proteins in SW1990 cells.The incidences of metastasis were decreased significantly in L-EMO group and H-EMO group as compared with that in control group.The percentage of CD34,NF-κB and MMP-9-positive cells in the tumors were significantly reduced by the administration of emodin.Conclusion： Emodin exerts anti-metastatic activity in pancreatic cancer both in vitro and in vivo,which may be related to down-regulation of NF-κB and MMP-9.

关 键 词：胰腺肿瘤 大黄素 转移 

分 类 号：R735.9[医药卫生—肿瘤]