“套管法”建立大鼠自体静脉移植模型及其病理学观察  

Pathological features of autologous vein graft model established by cuff technique in rats

作　　者：邹君杰[1] 章希炜[1] 杨宏宇[1] 马昊[1] 

机构地区：[1]南京医科大学第一附属医院血管外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第10期1533-1535,1540,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省人事厅留学回国人员基金(303070470I-A06)

摘　　要：目的:探讨"套管法"建立大鼠颈静脉-颈动脉移植模型的可行性,并观察移植静脉早期的病理学变化。方法:采用套管法建立SD大鼠颈静脉-同侧颈动脉移植模型。分别在静脉移植后1、7和21 d取材,HE染色观察移植静脉血管壁组织血管结构变化特点,Digimizer图像处理分析软件测量内膜中膜增生厚度。结果:在18只模型中2只动物死亡,1只发现移植静脉内血栓形成,总成功率为83.3%。术后1 d移植静脉内膜中膜厚度为(48.47±11.28)μm,7 d内膜中膜开始增厚至(66.05±19.10)μm,术后21 d增生更加明显至(300.44±181.54)μm,三者相比具有统计学差异(χ2=125.2,P<0.01)。1、7及21 d两两之间相比均具有统计学差异。结论:采用套管法建立的大鼠颈静脉-颈动脉移植模型方法简单,成功率高。其能较确切的复制人体静脉移植的病理生理过程,可应用于人类移植静脉狭窄闭塞的实验研究。Objective： To investigate the feasibility of establishmene of a reliable jugular vein-carotid artery transplantation rat model,and observe the pathological features of the vein grafts.Methods： The jugular vein-carotid artery transplantation model of SD rat was established by cuff technique.Grafts were harvested at 1 d,7 d,and 21 d,respectively,and underwent morphometric analysis by HE staining.The thickness of intimal media hyperplasia was measured by Digimizer image analysis software.Results： Eighteen vein graft animal models were established successfully.There were two rats died,and vein graft thrombosis at one animal.The overall success rate was 83.3%.The intimal media thickness of vein graft was（48.47 ± 11.28） μm after 1 d and increased to（66.05 ± 19.1） μm after 7 d,and increased to（300.44 ± 181.54） μm after 21 d（？字2 = 125.2,P 0.01）,and comparison between any two has a significant difference.Conclusion： The cuff technique of estabilish ment of vein graf model was simple and with high successful rate.The model can replicate the pathophysiology of human vein graft procedure exactly,consequently be widely used in experimental studies for human vein graft diseases.

关 键 词：自体静脉移植 内膜增生 动脉模型 大鼠 

分 类 号：R364.1[医药卫生—病理学]