水痘-带状疱疹病毒Oka疫苗株与野毒株序列比对  

Alignment of varicella-zoster virus sequences between Oka vaccine strain and wild-type strain

作　　者：陈哲文[1] 金于兰[2] 瞿爱东[3] 马相虎[4] 王亮[4] 

机构地区：[1]上海生物制品研究所奉贤开发办公室,200052 [2]上海生物制品研究所第七研究室,200052 [3]上海生物制品研究所第一研究室,200052 [4]上海生物制品研究所生物技术制品中心,200052

出　　处：《国际生物制品学杂志》2011年第5期230-236,共7页International Journal of Biologicals

摘　　要：目的观察水痘-带状疱疹病毒（varicella—zoster virus，VZV）Oka疫苗株（V—Oka株）的传代稳定性，并与分离于水痘患者的野毒株进行序列比对。方法将V—Oka株在人二倍体细胞MRC-5中连续传代培养至50代，提取32、34、38、42、46和50代V—Oka株及野毒株的DNA，对主要基因（ORF31、ORF62、ORF68、R区）进行PCR扩增和测序，并进行序列比对。结果与GenBank中的V—Oka株序列相比，32代至50代V-Oka株的ORF31、ORF62、ORF68、R区序列稳定，并趋于减毒。未发现野毒株序列发生如同V—Oka株的独特变异。结论连续传代培养至50代，V—Oka株的分子遗传学特性保持稳定。序列比对显示，水痘患者的致病毒株为野毒株，而非V—Oka株。Objective To observe the stability of varicella-zoster virus （VZV） Oka vaccine （V-Oka） strain during subculture, and align VZV sequences between V-Oka strain and wild-type strain from chickenpox patients. Methods V-Oka strain was subcuhured to the 50th passage on human diploid cell MRC-5. VZV DNAs were extracted from the 32rid, 34th, 38th, 42rid, 46th and 50th passages of V-Oka strains and wild-type strains. The major genes （ ORF31, ORF62, ORF68 and repeat regions） of V-Oka strains and wild-type strains were amplified by PCR and sequenced. DNA sequences between V-Oka strains and wild-type strains were aligned. Results In comparison with DNA sequences of V-Oka strain in GenBank, ORF31, ORF62 , ORF68, repeat regions sequences of V-Oka strains from the 32rid passage to the 50th passage were stable and seemed to be more attenuated. Unique mutations as same as those in V-Oka stains were not observed in wild-type strains. Conclusions The molecular genetic characteristics of V-Oka strains are stable till the 50th passage. The results of sequence alignment show that pathogenic strains in chickenpox patients are wild-type strains, but not V-Oka strains.

关 键 词：水痘疫苗 疱疹病毒3型 人 序列比对 

分 类 号：R3[医药卫生—基础医学]