骨癌痛大鼠脊髓背角T细胞死亡相关基因8表达的变化  

Chanse in T-cell death-associated gene 8 expression in spinal cord in rats with bone cancer pain

作　　者：殷伟[1] 杭黎华[1] 杨建平[1] 王丽娜[1] 郑小艳[1] 许期年[2] 王秀云[2] 左剑玲[2] 

机构地区：[1]苏州大学附属第一医院麻醉科,215006 [2]苏州大学附属第一医院脑神经研究室,215006

出　　处：《中华麻醉学杂志》2011年第8期935-937,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30872442,81000479）;江苏省卫生厅基金（H200917）

摘　　要：目的探讨骨癌痛大鼠脊髓背角T细胞死亡相关基因8（TDAG8）表达的变化。方法雌性未交配SD大鼠224只，体重150—180g，采用随机数字表法，将大鼠随机分为3组：正常对照组（Ⅰ组，n=64）、生理盐水组（Ⅱ组，n=64）和骨癌痛组（Ⅲ组，n=96）。采用左侧胫骨骨髓腔内接种WMker256乳腺癌肿瘤细胞的方法建立骨癌痛模型。Ⅲ组于接种前1天（基础状态）及接种后第t、3、6、9、12、15、18天、Ⅰ组和Ⅱ组于相应时点取8只大鼠，测定左后肢机械缩足阈值（MWT）。Ⅲ组于接种前1天（基础状态）及接种后第6、9、12、15、18天各处死16只，Ⅰ组和Ⅱ组相应于接种后第18天处死16只，取L4-6脊髓，免疫组化染色法测定脊髓背角TDAG8阳性细胞计数，实时，定量PCR法检测脊髓TDAG8mRNA的表达。结果与I组及基础值相比，Ⅲ组接种后第6～18天MWT降低，脊髓背角TD-AG8阳性细胞计数升高，脊髓rIDAG8mRNA表达上调（P〈0．01），Ⅱ组差异无统计学意义（P〉0．05）。结论骨癌痛大鼠脊髓TDAG8表达上调，该变化可能是骨癌痛形成的机制。Objective To investigate the changes in the expression of T-ceU death-associated gene 8（TD- AG8） in spinal cord in rats with bone cancer pain. Methods Two hundred and twenty-four female rats weighting 150-180 g were randomly divided into 3 groups： normal control group（group Ⅰ , n = 64）, normal saline group （group Ⅱ , n = 64） ,bone cancer pain group（group Ⅲ , n = 96）. Bone cancer pain was induced by inoculating Walker256 mammary gland carcinoma cells into the tibia medullary cavity. Mechanical withdrawl threshold（MWT） was measured at 1 d before （baseline） and 1,3,6,9,12,15 and 18 d after inoculation. Sixteen rats were sacrificed at 1 day before（baseline）and 6,9, 12, 15 and 18 d after inoculation in group m and 18 d after inoculation in groups Ⅰ and Ⅱ . The L4-6 spinal cord were removed, and the number of TDAG8 positive cell was counted, and the expression of TDAG8 mRNA was measured by RT-PCR. Results Compared with baseline value and group Ⅰ , MWT was decreased, and the number of TDAG8 positive cells and the expression of TDAG8 mRNA in spinal cord were increased at 6-18 d after inoculation in group m （ p 〈 0.01 ）. Conclusion The expression of TDAG8 in spi- nal cord is up-regulated in rats with bone cancer pain, which may be involved in the mechanism of the development of bone cancer pain.

关 键 词：受体 G-蛋白偶联 骨肿瘤 疼痛 脊髓 

分 类 号：R738.1[医药卫生—肿瘤]