蛛网膜下腔注射R025—6981对大鼠局灶性脑缺血再灌注损伤的影响  

Effects of lntrathecal Ro25-6981 onfocal cerebral ischemia-reperfusion injury in rats

作　　者：闫芳[1] 张艳兵[1] 王丽娜[1] 杨建平[1] 詹英[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,215006

出　　处：《中华麻醉学杂志》2011年第8期977-979,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30872442,81000479）

摘　　要：目的评价蛛网膜下腔注射R025—6981对大鼠局灶性脑缺血再灌注损伤的影响。方法清洁级健康雄性SD大鼠60只，体重280—320g，采用随机数字表法，将其随机分为4组（n=15）：假手术组（S组）、脑缺血再灌注组（I／R）、生理盐水组（Ns组）和选择性NR2B受体拮抗剂R025．6981组（Ro组）。采用线栓法阻塞右侧大脑中动脉2h恢复灌注的方法制备大鼠局灶性脑缺血再灌注损伤模型。Ro组于再灌注即刻、再灌注2、23h时蛛网膜下腔分别注射R025-6981100μg／10m，NS组注射等体积生理盐水。于再灌注24h时行神经功能缺陷评分，随后处死大鼠取脑，采用2，3，5-氯化三苯基四氮唑法测定脑梗死体积。结果与S组比较，I／R组、Ns组和Ro组神经功能缺陷评分升高，脑梗死体积增加（P〈0．01）；与I／R组比较，Ro组神经功能缺陷评分降低，脑梗死体积减少（P〈0．01），NS组上述指标差异无统计学意义（P〉0．05）。结论蛛网膜下腔注射R025．6981可减轻大鼠局灶性脑缺血再灌注损伤。Objective To investigate the effect of intratheeal （IT） Ro25-6981 （a selectiye NR2B receptor antagonist） on focal cerebral isehemia-reperfusion （I/R） injury in rats. Methods Sixty healthy male SD rats weighing 280-320 g in which IT catheter was successfully implanted without complication were randomly divided into 4 groups （ n = 15 each） ： group sham operation （group S） ; group focal cerebral I/R（group I/R） ; group I/R ＋ Ro25-6981 （group Ro） and group I/R ＋ normal saline （group NS）. The right middle cerebral artery was occluded for 2 h with a nylon thread with rounded tip which was inserted into right internal carotid artery and advanced cranially until resistance was met in groups I/R,Ro and NS. In group Ro Ro25-6981 100 μg/10 IA was injected IT at 0, 2,23 h of reperfusion, while in group NS NS was injected IT instead of Ro25-6981. Neurological dificit was as- sessed and scored （0 = no deficit, 3 = severe deficit） at 24 h of reperfusion. The infarct size was determined by 2, 3, 5-triphenyl tetrazolium chloride staining. Results IT administration of Ro25-6981 significantly reduced cerebral I/R-induced infarct sized and neurological deficit score in group Ro as compared with groups I/R and NS. Conclusion Ro25-6981 injected IT can protect the brain against local cerebral I/R injury.

关 键 词：受体 N-甲基-D-天冬氨酸 注射 脊髓 再灌注损伤 脑 

分 类 号：R743.3[医药卫生—神经病学与精神病学]