血管内皮生长因子C在卵巢上皮癌组织内的表达及其预后意义  

Prognostic significance of vascular endothelial growth factor C expression in epithelial ovarian carcinoma

作　　者：张靖[1,2] 肖建兵[1] 李雪梅[1] 李鑫磊[1] 马晶[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150081 [2]哈尔滨市第五医院骨科,黑龙江哈尔滨150040

出　　处：《解剖科学进展》2011年第6期538-540,544,共4页Progress of Anatomical Sciences

基　　金：黑龙江省自然科学基金资助项目(No.D201041)

摘　　要：目的观察血管内皮生长因子(VEGF)-C在卵巢上皮癌组织内的表达,分析VEGF-C的表达与癌临床病理特征和预后之间的关系,为临床抗淋巴管生成治疗肿瘤及评价患者预后提供理论依据。方法取人卵巢上皮癌组织蜡块78例和术后卵巢癌组织12例,免疫组化法和Western blot法观察VEGF-C在卵巢上皮癌组织内的表达情况。结果免疫组化结果显示VEGF-C蛋白主要表达于卵巢癌细胞胞浆内,VEGF-C在淋巴结转移组卵巢癌组织内的表达阳性率明显高于无淋巴结转移组(<0.01)。Western blot结果可见伴淋巴结转移卵巢癌组织内VEGF-C的表达水平高于无淋巴结转移卵巢癌组织。Kaplan-Meier和Cox回归分析表明,VEGF-C表达阴性患者的五年总体生存率和无瘤生存率均高于表达阳性患者。结论 VEGF-C表达与卵巢上皮癌淋巴结转移密切相关,可以作为判断卵巢上皮癌患者预后的新指标。Objective To investigate the relationship of vascular endothelial growth factor C（VEGF-C）with clinicopathological characteristics and prognosis,we observed the expression of VEGF-C in human epithelial ovarian carcinoma.Methods Seventy-eight paraffin-embedded stored specimens and 12 postoperation tissue from patients with epithelial ovarian carcinoma with pathological diagnosis were included in this study.The expression of VEGF-C was detected by immunohistochemical stain and Western blot.Results VEGF-C protein expressed in cytoplasm of malignant cells by immunohistochemical stain,the positive rate of VEGF-C protein expression in lymph node metastatic ovarian carcinoma was significantly higher than that in nonmetastatic group（P〈0.01）.VEGF-C protein expression level was also detected higher in ovarian cancer with lymph node metastasis than in ovarian carcinoma without lymph node metastasis by Western blot.Kaplan-Meier and Cox proportional hazards model analyses indicated that 5-year overall survival rate and disease-free survival rate in VEGF-C negative expression patients was higher than that in positive expression patients.Conclusion VEGF-C expression closely correlates with lymph node metastasis in epithelial ovarian carcinoma,and is an independent factor for evaluating the epithelial ovarian carcinoma prognosis.

关 键 词：人卵巢上皮癌 VEGF-C 淋巴结转移 预后 

分 类 号：R737.31[医药卫生—肿瘤]