急性白血病患者WIF-1基因启动子甲基化及β连环蛋白表达的研究  

Study of WIF-1 promoter methylation with expressions of β-catenin in acute leukemia

作　　者：王焱[1] 朱传升[1] 毕可红[1] 徐文伟[1] 董琳[1] 侯明[2] 

机构地区：[1]山东大学附属千佛山医院血液科,济南250014 [2]山东大学齐鲁医院血液科

出　　处：《中华医学杂志》2011年第40期2858-2860,共3页National Medical Journal of China

基　　金：基金项目：山东省科技攻关项目（2006GG3202037）

摘　　要：目的探讨急性白血病（AL）患者Wnt抑制因子1（WIF-1）基因启动子甲基化及β连环蛋白（β-catenin）的表达在白血病中的意义。方法采用甲基化特异性PCR检测山东大学附属千佛山医院2009年1月至2010年6月55例AL患者及对照组骨髓单个核细胞WIF-1基因启动子甲基化情况，流式细胞术检测AL患者及对照组骨髓单个核细胞β—catenin表达。结果32．7％（18／55）的AL患者WIF-1基因启动子区域有异常甲基化，对照组未检测到WIF-1基因甲基化，两组差异有统计学意义（P〈0．05）。甲基化组首次诱导治疗完全缓解率（38．9％，7／18）低于非甲基化组（81．1％，30／37），差异有统计学意义（P〈0．05）。甲基化组与非甲基化组β—catenin的表达（17．5％±3．3％、15．4％±3．6％）均高于对照组（10．5％±1．5％，均P〈0．05）；甲基化组β—catenin的表达高于非甲基化组（P〈0．05）。结论WIF-1基因启动子甲基化及β—catenin过表达可能参与了AL患者Wnt／β-catenin途径的异常激活。Objective To explore the significance of WIF-1 gene promoter methylation and the expression of β-catenin in acute leukemia （AL） patients. Methods The method of methylation specific polymerase chain reaction was employed to detect the status of WIF-1 gene methylation in 55 acute leukemia patientsand normal controls from January 2009 to June 2010 in our hospital. The expression of β-eatenin was measured by flow cytometry. Results The methylation of WIF-1 gene promoter was found in 32. 7% （18/55） AL patients. And the percentage was significantly higher than that of the controls （0）. The patients with the methylation of WIF-1 gene had a lower complete remission rate（38.9% ,7/18）for the first chemotherapy than those without （ 81.1% ,30/37 ） （ P 〈 0. 05 ）. The expressions of β-catenin in methylation AL patients and those with non-methylation were 17.5% ± 3.3% and 15.4% ± 3.6% respectively. And they were significantly higher than the controls （ 10. 5% ± 1.5% , P 〈 0. 05 ）. The expression of β-catenin was higher in positive methylation patients than those negative ones （P 〈 0. 05 ）. Conclusion The methylation of WIF-1 gene promoter and β-catenin may be involved in the abnormal activation of Wnt/β-catenin signal in acute leukemia.

关 键 词：白血病 DNA甲基化 Β连环素 WIF-1基因 

分 类 号：R733.71[医药卫生—肿瘤]