两型星形胶质细胞损伤后胰甘油三酯脂酶的表达  

Expression of pancreatic triglyceride lipase in injured primary type-1 and type-2 astrocytes

作　　者：赵俊利[1] 张晔[1] 刘凯[1] 孙艳芸[1] 贾俊霞[1] 夏春林[1] 

机构地区：[1]苏州大学医学部博习临床解剖学研究所暨细胞神经生物学研究室,苏州215123

出　　处：《神经解剖学杂志》2011年第5期481-486,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30772242)

摘　　要：目的:通过建立两型星形胶质细胞体外机械损伤模型,于损伤后不同时间点检测两型星形胶质细胞中胰甘油三酯脂酶(PTL)的表达变化。方法:采用震荡培养和差速贴壁法分离纯化新生SD大鼠的1型星形胶质细胞(T1As)和2型星形胶质细胞(T2As),以real-time PCR和Western Blot分别检测损伤后的两型星形胶质细胞中PTL mRNA和蛋白表达变化。结果:损伤后两型星形胶质细胞中PTL表达均出现先升高后降低的趋势,而在T2As中的表达远高于在T1As中的表达水平(P<0.01)。结论:PTL在T2As中高表达提示,中枢神经系统损伤后再生与修复过程中,T2As对脂质代谢及髓鞘形成发挥作用。Objective： To establish the two types of astrocytes injury model in vitro and detect the expression of pancreatic triglyceride lipase in injured primary type-1 astrocytes（T1As） and type-2 astrocytes（T2As） in different times.Methods： Purified cultures of T1As and T2As were prepared from neonatal rats,based on the differential properties of developmental time-course and cellular adhesions.The expression of pancreatic triglyceride lipase after astrocytes injured in the level of mRNA and protein was examined by real-time PCR and Western Blot.Results： The expression of PTL both in the levels of mRNA and protein began to increase 6 h after mechanical injury of astrocytes and reached the peak at 24 h,compared with the control group（P0.01）,and then declined.Furthermore,the expression in T2As was significantly higher than that in T1As.Conclusion： High expression of PTL in T2As suggests that T2As are closely correlated with myelogenesis and metabolism of lipids in the repair and regeneration of central nervous systerm following injury.

关 键 词：星形胶质细胞 胰甘油三酯脂酶 细胞损伤 神经损伤与修复 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]