不同年龄段前列腺外周带基质细胞对上皮细胞生长特性的影响  

Effect of prostatic stromal cells from varying ages on the growth of prostate epithelial cells

作　　者：王永传[1] 韩邦旻[1] 赵福军[1] 王小海[1] 洪艳[1] 夏术阶[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,200080

出　　处：《中华实验外科杂志》2011年第12期2057-2060,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（81072096）;上海市教育委员会科研创新重点项目（102216）

摘　　要：目的观察不同年龄段前列腺外周带基质细胞对上皮细胞体内、外生长特性的影响。方法原代培养年轻组（20—30岁）和老年组（55～80岁）前列腺外周带基质细胞各5例，利用微孔双室共培养系统将前列腺上皮细胞BPH-1（或PC-3）与基质细胞共培养，观察基质细胞对BPH-1及PC-3细胞体外增殖、迁移和侵袭能力的影响。裸鼠皮下混合种植BPH-1（或PC-3）与外周带基质细胞，通过绘制成瘤曲线和瘤体组织形态学分析来比较老年组和年轻组外周带基质细胞对上皮细胞体内成瘤的影响。结果与年轻组外周带基质细胞比较，老年组基质细胞具有更强的促前列腺上皮细胞BPH-1（或PC-3）体外增殖、迁移和侵袭能力（P〈0．01）；体内成瘤实验提示，老年组外周带基质细胞具有更强的促PC-3细胞成瘤生长和促高代数（〉100代）的BPH-1细胞恶性进展的能力（P〈0．05）。结论老龄化前列腺外周带基质细胞特性的改变可能是老年男性前列腺癌（PCa）高发和恶性进展的原因之一。Objective To explore the influences of human prostatic stromal cells cultured from prostatic peripheral zone of young donors （PZ-young） and old donors （PZ-old） on prostate epithelial cells. Methods We established primary prostatic stromal cell cultures from prostatic peripheral zone of young donors （20-30 years old） and old donors （55-80 years old）, and co-cultured these stromal cells with prostate epithelial cells （ immortalized non-tumorigenic BPH-1 cells and tumorigenic invasive PC-3 cells） in two-chamber coculture system. The proliferation, motility and invasive ability of these epithelial cells were assessed. We also co-transplanted stromal cells with BPH-1 （ or PC-3） cells subcutaneously into nude mice to compare their influence on epithelial tumor growth. Results As compared with the PZ-young group, both BPH-1 or PC-3 cells grew faster when cocultured with PZ-old ceils in vitro （P 〈 0. 01 ）. Stromal cells from PZ-old markedly triggered the migration of both PC-3 and high passage BPH-1 cells （ 〉 100 passage）, and promoted the invasion of PC-3 cells in vitro （ P 〈 0. 05 ）. Using in vivo tissue recombination model, we also found the PZ-old stromal cells reduced ＂tumor＂ latency by BPH-1 cells and promoted the tumor progression by PC-3 cells significantly compared to PZ-young cells （ P 〈 0. 01 ）. Conclusion The PZ-old prostate stromal cells could stimulate the proliferation, migration and invasion of PC-3 and BPH-1 cells in vitro and enhance their malignant progression in vivo.

关 键 词：前列腺 外周带 基质细胞 共培养 年龄 

分 类 号：R737.25[医药卫生—肿瘤]