血管内皮生长因子-C在食管鳞癌中的表达及临床意义  

Expression of vascular endothelial growth factor C in esophageal carcinoma and its clinical significance

作　　者：尹玉慧[1] 李向龙[2] 张红新[1] 张岚[1] 陈奎生[1] 张云汉[1] 

机构地区：[1]郑州大学第一附属医院病理科河南省肿瘤病理重点实验室,450052 [2]河南省郏县人民医院

出　　处：《中华实验外科杂志》2011年第12期2186-2189,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：河南省基础与前沿技术研究项目（102300410078）;河南省重点科技攻关项目（112102310088）

摘　　要：目的探讨血管内皮生长因子-C（VEGF—C）在食管鳞癌中表达及临床意义。方法应用免疫组织化学和原位杂交技术检测VEGF—C在食管鳞癌组织中的表达及其与淋巴结转移的关系。转染VEGF—C小干扰RNA（siRNA）质粒于食管癌EC9706细胞，利用逆转录-聚合酶链反应（RT-PCR）和Westernblot检测siRNA对VEGF—C表达的抑制特征，并检测裸鼠体内成瘤实验瘤组织中VEGF—C表达。结果VEGF—C在食管鳞癌有无淋巴结转移组表达率分别为90．0％和62．1％，与淋巴结转移密切相关（P〈0．01）；转染组VEGF—C蛋白表达灰度比值为0．32±0．02，较无关序列转染对照组表达降低（0．91±0．09），差异均有统计学意义（P〈0．01）；裸鼠移植瘤组织中VEGF—C阳性率分别为9．26±0．95、8．94±0．81、9．19±0．92，较对照组表达降低（32．47±3．25），差异均有统计学意义（P〈0．01）。结论VEGF-CsiRNA在体内外均能有效地抑制VEGF—C的表达。Objective To investigate the expression of vascular endothelial growth factor C （VEGF-C） in human esophageal squamous cell carcinoma （ESCC） and its clinical significance. Methods The expression of VEGF-C in ESCC and its correlation with lymph node metastasis were detected by using immunohistochemistry （IHC） and in situ hybridization （ISH）. Three small interfering RNA （siRNA） expression vectors targeting VEGF-C were constructed and transformed into EC9706 cells. The inhibitory effect of siRNA on the expression of VEGF-C protein and mRNA in tranfected cells was analyzed by using Western blotting and reverse transcription-polymerase chain reaction （RT-PCR）. Stably transfected cells were transplanted in nude mice later. The expression of VEGF-C in transplant tumor was detected by using IHC and ISH. Results VEGF-C was up-expressed in ESCC. Its expression was correlated significantly with lymph node metastasis. The positive expression rate of VEGF-C in lymph node metastasis group and non-lymph node metastasis group was 90. 0% and 62. 1% respectively （P 〈 0. 01 ）. VEGF-C-targeted siR- NA could decrease the expression of VEGF-C mRNA and protein in EC9706 cells effectively. There was significant difference between transfected group （0. 32 ± 0.02 ） and un-transfected group （0. 91 ± 0.09, P 〈0. 01 ）. Correspondingly, the expression of VEGF-C was 9. 26 ±0. 95, 8.94 ±0. 81, 9. 19 ±0. 92 and 32.47 ± 3.25 respectively. There was significant difference among them （P 〈 0. 01 ）. Conclusion Targeting VEGF-C siRNA can inhibit VEGF-C expression of EC9706 in vivo and in vitro effectively.

关 键 词：食管癌 血管内皮生长因子-C 

分 类 号：R735.1[医药卫生—肿瘤]