抑制性消减杂交技术在器官纤维化中的研究进展  

PROGRESS OF SUPPRESSION SUBTRACTIVE HYBRIDIZATION IN ORGAN FIBROSIS

作　　者：侯文娜[1] 马小兵[1] 王献华[1] 

机构地区：[1]河北联合大学病理学教研室,唐山063000

出　　处：《现代预防医学》2011年第23期4924-4926,4929,共4页Modern Preventive Medicine

基　　金：河北省科学技术研究与发展计划项目(09276192D);河北省自然科学基金资助项目(C2008001007)

摘　　要：抑制性消减杂交技术(SSH)是一种简便易行、特异性高的高效分离差异表达基因的方法。本文简要介绍了SSH的基本原理及过程,并详细论述了其在多种器官纤维化中的研究进展。通过SSH,可获得大量与器官纤维化相关的基因,有助于进一步阐明器官纤维化的发生机制,探究有效的防治措施。Suppression subtractive hybridization（SSH）,which is feasible and highly specific,is one of the most efficient methods for cloning differentially expressed genes.In this paper,we reviewed the principle and procedure of SSH briefly and discussed the current research progress of SSH in various organ fibrosis in detail.A lot of genes related to organ fibrosis were cloned by SSH,which could bring light to the explanation of the pathogenesis of organ fibrosis and the exploration of effective measures of prevention and treatment.

关 键 词：抑制性消减杂交 差异表达基因 CDNA消减文库 

分 类 号：R394.3[医药卫生—医学遗传学]