50Hz正弦电磁场对骨肉瘤MG-63细胞增殖的影响  

The effect of 50 Hz sinusoidal electromagnetic fields on the proliferation of the osteosarcoma cell line MG-63

作　　者：尚林[1] 刘阳[1] 宋明宇[1] 虞冀哲[1] 吴华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院矫形外科,武汉430030

出　　处：《中华物理医学与康复杂志》2011年第11期812-814,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金项目（51077065）

摘　　要：目的探讨50Hz、1．0mT正弦电磁场对骨肉瘤MG-63细胞增殖的影响。方法将骨肉瘤MG-63细胞分成对照组和实验组，对照组放在无电磁场的培养箱中，实验组放入含有50Hz、1．0mT正弦电磁场的培养箱中，分别在第2天、第4天和第6天采用细胞计数试剂盒-8（CCK-8）法检测细胞增殖活性，流式细胞仪测定细胞周期，逆转录-聚合酶链反应（RT—PCR）技术检测cyclin B1和cyclin D1的mRNA表达水平变化。结果2组比较，实验组细胞增殖活性明显降低、G0-G1期细胞数目增多、cyclin B1和cyclin D1的mRNA表达水平下降。结论50Hz、1．0mT正弦电磁场能够显著抑制骨肉瘤MG-63细胞增殖。Objective To investigate the biological effect of 50 Hz sinusoidal electromagnetic fields at 1 mT on the proliferation of the human osteosareoma cell line MG-63. Methods Osteosarcrma MG-63 cells were divided into control and experimental groups. The control group was incubated without an electromagnetic field; the experimental group was incubated in a 50 Hz, 1. 0 mT sinusoidal electromagnetic field. On the 2nd, 4th and 6th day, their proliferation was determined using a cell counting kit-8 （CCK-8） assay. Variations in the cell cycle were detected with flow cytometry （FCM）. Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） was used to measure cyclin B1 and cyclin D1 mRNA. Results Compared with the control group, proliferation of the experimental group cells was reduced significantly. The percentage of cells at G0-G1 phase increased, and the mRNA expression of cyclin B1 and cyclin D1 was significantly reduced. Conclusions A 50 Hz sinusoidal electromagnetic field at 1. 0 mT can inhibit the proliferation of osteosarcoma cell line MG-63 significantly.

关 键 词：正弦电磁场 骨肉瘤细胞 增殖 细胞周期 

分 类 号：R73[医药卫生—肿瘤]