广西地区人群XPF基因RS744154多态性与胃腺癌发病风险及其临床病理特征的关系  

Relationship between the XPF gene RS744154 polymorphism and risk of gastric carcinoma and its clinicopathololgic features among guangxi area

作　　者：王名法[1] 王超[2] 马韵[1] 邓卓霖[1] 黄永秩[3] 王华[1] 梁秀就[1] 廖文莉[1] 龙喜带[3] 

机构地区：[1]广西医科大学病理学教研室,南宁530021 [2]右江民族医学院附属医院消化内科,百色533000 [3]右江民族医学院病理学教研室,百色533000

出　　处：《临床与实验病理学杂志》2011年第11期1169-1172,1176,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：广西青年科学基金(桂科青0832097);广西科技攻关项目(桂科计字[2008]98号)

摘　　要：目的探讨广西地区人群XPF基因RS744154多态性与胃腺癌发病风险及其临床病理特征的关系。方法应用Taq-Man-PCR方法对99例胃腺癌患者及284例正常人群的XPF基因RS744154多态性进行检测,并分析各基因型与胃腺癌患者临床病理特征的关系。结果 (1)XPF基因3种基因型(即XPF-GG、-GC和-CC型)在胃腺癌组与对照组中的分布差异具有统计学意义,以XPF-GG基因型为参照,其中携带XPF-CC者胃腺癌的发病风险增加(CC对应比值比OR为2.60);(2)分层分析显示,带风险基因型(即XPF-GC/CC)的男性、汉族或高龄者发病风险分别是不带风险基因型者的2.25倍、1.95和3.00倍(P<0.05);(3)XPF-GG、GC/CC基因型在肿瘤直径≤5 cm和>5 cm患者中出现频率分别为43.75%、71.64%和56.25%、28.36%,两者差异有统计学意义(χ2=7.198,P=0.007,OR=3.69);(4)XPF基因多态性与胃腺癌患者肿瘤发生部位、浸润深度、病理分级、Laurén分类、淋巴结转移和TNM分期均无相关性;(5)HP感染发生率在不同基因型胃腺癌患者中差异无统计学意义。结论 XPF基因RS744154多态性与广西人群胃腺癌发病风险相关,在男性、汉族及高龄者更为明显。癌旁胃黏膜HP感染与XPF基因RS744154多态性之间无显著相关性,同时其多态性也与影响胃腺癌预后的临床病理特征无相关性。Purpose To explore the relationship between the XPF gene RS744154 polymorphism and risk of gastric carcinoma（GAC） and its clinicopathologic features among Guangxi area.Methods This study,including GACs（n=99） and control group（n=284）,was carried out to analyze XPF RS744154 polymorphism using TaqMan-PCR technique.Then association analyses was performed between these polymorphism sites and the clinicopathologic features of GAC.Results We found the distribution of three XPF genotypes（namely XPF-GG,-GC,and-CC） were found in GAC was significantly different from the controls（P0.05）.Compared with GG genotype,CC can increase the risk of GAC（corresponding OR was 2.60 for XPF-CC）.In the stratified analysis,we also observed that males、The Han nationality or elders having risk genotypes（OR1,also called XPF-GC/CC） which will increase the risk of GAC（OR was 2.25、1.95 and 3.00,respectively） compared to those without risk genotypes.The frequencies of the XPF-GG,GC/CC genotypes were 43.75%,71.64% in patients with tumor diameter（≤ 5 cm）,and 56.25%、28.36% in patients with tumor diameter（5 cm）,respectively.The difference was statistical significance（χ2=7.198,P=0.007,OR=3.69）.No relationship was found between XPF gene polymorphisms and tumor sites,pathologic grade,Laurén classification,lymph nodemetastasis,TNM stage.The distribution of HP infection among three XPF genotypes（namely XPF-GG,-GC,and-CC） was not significantly difference in GAC（P0.05）.Conclusion These results suggest that XPF RS744154 polymorphism may associate with the risk of gastric adenocarcinoma among Guangxi population,especially among males the Han nationality and elders.The distribution of HP infection in the paracancerous mucosa and the XPF gene RS744154 polymorphism is not significantly difference.Furthermore,XPF gene polymorphism also may not correlation with the clinico-pathologic feature.

关 键 词：胃腺肿瘤 XPF 基因多态性 SNP 

分 类 号：R735.2[医药卫生—肿瘤]