高良姜素诱导肺癌A549细胞凋亡的研究  

Galangin Induces Apoptosis on Lung Cancer A549 Cells

作　　者：伍俊[1] 文敏[2] 张海涛[2] 

机构地区：[1]广东医学院呼吸疾病研究所,广东湛江524023 [2]广东医学院生物化学与分子生物学教研室

出　　处：《肿瘤防治研究》2011年第11期1228-1231,共4页Cancer Research on Prevention and Treatment

基　　金：广东省科技计划资助项目(00124381120302036);东莞市科技计划资助项目(200910815258);广东省中医药管理局资助项目(2010190)

摘　　要：目的研究高良姜素诱导肺癌细胞系A549细胞凋亡效应。方法 MTT法测定A549细胞生长抑制率,荧光显微镜观察细胞形态学的变化,流式细胞仪分析细胞周期和线粒体膜电位的变化。Western blot检测细胞凋亡相关蛋白的表达变化。结果随高良姜素浓度增高,A549细胞生长抑制率明显上升,IC50在30.15 mg/L。细胞凋亡可在10～80 mg/L高良姜素处理后24 h出现,呈浓度依赖性。高良姜素使线粒体膜电位降低。Caspases被激活,Bcl-2、Bcl-XL表达下调,p53、Bax、Bid表达呈浓度依赖性上调。结论高良姜素可能是通过线粒体途径诱导A549细胞发生凋亡。Objective To investigate apoptosis induced by Galangin in lung cancer A549 cells.Methods Cell inhibition ratio was assayed by MTT method.Morphologic assessment of apoptosis was performed with a fluorescence microscope,and cell cycle and mitochondrial membrane potential were analyzed by flow cytometry.The expression levels of proteins were measured by Western blot.Results A549 cells were inhibited by Galangin in a dose-dependent manner.IC50 of Galangin was 30.15 mg/L.The apoptosis appeared in 24 hours after treated by Galangin.Morphologic changes including nuclear chromatin condensation fluorescence strength was observed through fluorescence microscope.Caspases were activated.The expression of Bcl-2 and Bcl-XL decreased,while the expression of p53,Bax and Bid increased.Conclusion Galangin could induce A549 cell apoptosis through mitochondria pathway.

关 键 词：高良姜素 A549 荧光 细胞凋亡 线粒体膜电位 

分 类 号：R734.2[医药卫生—肿瘤]